Abstract: PCR-RFLP technique was used to analyze the relationship between the polymorphisms of IGFBP3 gene and beef performance in 24 Luxi cattles and 19 Jinnan cattles. The results showed that there were polymorphisms in this locus, but only AA and AB genotypes. The genotype frequencies were 0.875/0.125 and 0.895/0.105 respectively. A and B gene frequencies were 0.938/ 0.062 and 0.948/0.052 respectively. Correlations between IGFBP3 genotypes and 19 beef performances were analyzed, and other indexes of individuals with genotypes AB were higher than those of AA except carcass depth, daily gain, net meat percentage, dressing percentage in Luxi cattle, which had no significant difference; While other indexes of individuals with genotypes AA were higher than those of AB except dressing percentage, bone weight, backfat thickness, carcass chest depth, ham girth, ham width, ham length, loin meat thickness in Jinnan cattle, which were insignificant.