Effect of Enucleation Time on Nuclear Transfer with Cumulus Cells in Mouse

Abstract

Abstract: Mice oocytes were enucleated during 14~16, 16~18, 18~20 and 20~22 h apart after injected hCG (Human Chorionic Gondaotropin, hCG), so as to research effect on mice somatic cell nucear transfer about enucleation time. Results show that encleation rate of mice oocytes was 80.09 %(338/422), 73.12 %(283 / 387), 55.02 %(230/418), and 48.74 %(193/396) respectively; Activation rate of embryos reconstructed was 66.20 %(190/287), 75.43 %(175 /232), 84.78 %(156/184), 85.14 %(126/148) apart; Blastocyst rate of embryos reconstructed was 13.94 %(40/287), 7.33 %(17/232), 3.80 %(7/184) and 1.35 %(2/148) respectively. It is indicated that enucleation time of mice oocytes is good during 14~16 h after injected hCG.

Cao Hongguo, Zhang Mingfeng, Zhang Yong. Effect of Enucleation Time on Nuclear Transfer with Cumulus Cells in Mouse[J]. Chinese Agricultural Science Bulletin, 2005, 21(3): 23-23.

References

1 Wilmut I,Schnieke A E,MeWhir J,et al.Viable offspring derived from fetal and adult mammalian cells. Nature, 1997, 385:810~813
2 Wakayama T,Perry A C,Zuccotti M,et al.Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei.Nature,1998,394:369~374
3 Baguisi A,Behboodi E,Melican DT,et al.Production of goats by somatic cell nuclear nuclear transfer.Nature Biotech, 1999,17:456~461
4 Kubota C,Yamakuchi H,Todoroki J,et al.Six cloned calves produced from fetal fibroblast cells after long-term culture. Proc Natl Acad Sci USA,2000,97:990~995
5 Polejaeva I A,Chen S H,Vaught T D,et al.Cloned pigs produced by nuclear transfer from adult somatic cells. Nature,2000,407:86~90
6 Stice S L,Robl J M.Nuclear reprogramming in nuclear transplant rabbit embryos.Biol Reprod,1988,39:657~664
7 Collas P,Robl J M.Factors affecting efficiency of nuclear transplantation in the rabbit embryo. Biol Reprod,1990,43:877~884
8 Collas P,Robl J M.Age of the recipient oocyte for nuclear transplantation in the rabbit.Biol Reprod,1989,40(suppl 1):110
9 Ware C B,Barnes F L,Maiki-Laurila M,et al.Age dependence of bovine oocyte activation. Gamete Res,1989,22:265~275
10 Robl J M,Stice S L.Prospects for the commercial cloning of animals by nuclear transplantation.Theriogenology,1989,31: 75~84
11 麜洪武,李荣凤,旭日干.提高牛体外受精核移植胚胎发育率的研究.动物学杂志,1995,30(6):39~43
12 Fulka J J,First N L,Moor R M.Nuclear transplantation in mammals: remolding of transplanted nuclei under the influence of maturation promoting factor. Bio Essays, 1996, 18(10):835~840
13 Campbell K H S,Ritchie W A,Wilmut I.Nuclear- cytoplasmic interactions during the first cell cycle of nucear transfer reconstructed bovine embryos: implications for deoxyribonucleicacid replication and development. Biol Reprod,1993,49:933~942
14 Collas P,Robl J M.Relationship between nuclear remodeling and developmention nuclear transplant rabbit embryos.Biol Reprod,1991:455~465
15 Trefil P,Horska M,Kanka J,et al.Response of avian nuclei to mammalian maturation promoting factor(MPF).Zygote, 1995,3(4):289~294
16 Kim JM,Ogura A,Nagata M,et al.Analysis of the Mechanism for Chromatin Remodeling in Embryos Reconstructed by Somatic Nuclear Transfer.Biol Reprod, 2002,67:760~766