Abstract:

Abstract: To research the goat pox virus by RNAi, the RNA polymerase 30 gene (RPO30) as a target sequence was analyzed. In the study, RPO30 was cloned by PCR, and sequencing shows that the open reading frame is 585bp that can code 193 amino acids,and there is a HindIII enzyme cut sites located during 110 to 117bp. The RPO30 sequence we cloned has different homology between different pox virus strains in the GenBank database. More bio-informatics software analyzed show that the amino acids site 4～12aa、18～26aa、50～61aa、68～92aa and176～190aa have more possibility of forming enzyme activity center. So, we should select these sites to inference.