Abstract:

This study was aimed to obtain higher efficiency in gene transfection into Luchuan Pig Fetal Fibroblasts and to study the role of green fluorescence proten (GFP) as a reporter system. We investigated the effect of parameter such as the dose of DNA and liposome number of cell transfected and exposure time of the cell to the DNA-liposome complexes. It was indicated that 6ug DNA with 15uL liposome resulted in the highest transfection efficiency after 8h transfection