Abstract:

To establish a yeast Pichia pastoris expression system expressing canine parvovirus VP2 protein (CPV VP2) to serve the diagnosis of CPV,development of CPV vaccine and research of VP2 protein function. To test the structure and fuction of VP2 protion in diagnoses and immunization in CDV.A pair of primers was designed and synthesized based on Canine parvovims(CPV)from GenBank,and the VP2 gene of CPV was cloned from bloody stool of a dead dog by PCR. The results showed that the complete sequence of the VP2 gene was 1 755 bp in ength . The nucleotide homology respectively is in 98.7%-99.7%.Then the VP2 gene was expressed by expression vector in BL21. The expressed productions were detected by SDS-PAGE and Western-blotting,and the results showed that the VP2 protein was high-efficiently expressed in E. coli.