Abstract:

Nodal explants derived from ancient mulberry (Morus alba), which was lived in the Summer Palace for more than 100 years, were used to initiate shoot cultures. Bud formation on the nodal explants was induced on an optimal medium MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+PVP 0.2g/L. To get a high propagation ratio, the seedlings were transferred to the medium of MS+ 6-BA 0.8 mg/L +NAA 0.1 mg/L +PVP 0.2 g/L. The optimal medium for adventitious root inducing was 1/2MS+NAA 0.5 mg/L +PVP 0.2 g/L, and the rooting rate was 100%. The plantlets were transplanted to pots with vermiculite-perlite mixture-humus soil (1:1:1) mixture and acclimated for several weeks. The rooted and acclimated plantlets were transferred outdoors with 95% transplantation success.