Abstract:

In this study，strain JM-11 with antagonistic activity against E. coli was isolated from the cecum of healthy chicken. As the morphology characteristics and physiological and biochemical characteristics of the strain JM-11 was finally identified as Bacillus subtilis subsp. subtilis. The test result of the tolerance in gastrointestinal tract and Bile salts were showed as follows: the strain JM-11 can survive in the simulated natural (bile salts content 0.3%, pH3.0 artificial gastric juice)environment. The model of diarrhea was produced by using antibiotics to break flora balance of intestinal tract, then pathogenicity E.coli was fed. The result of curing diarrhea of chicken by strain JM-11 was showed as follows: the quantity of E.coli was remarkably decreased (P<0.01), Lactobacilli activities significantly increased (P<0.01), immune organ index of chicken was significantly increased(P<0.05).