Abstract:

The liquid spawn medium formula and culture conditions of Pleurotus eryngii in submerged culture with shake flask were studied according to the index of mycelia biomass. The results showed that the optimum formula for Pleurotus eryngii liquid spawn medium was as follows: sucrose 3%, corn meal 3%, wheat bran 3%, yeast powder 0.4%, KH2PO4 0.25%, MgSO4 0.1%, ZnSO4 0.05%, CaSO4 0.05% and initial pH 6-7. The optimal culture condition: Volume 80 mL/250 mL, shaking rate 150 r/min, inoculation rate 10% and 6 culture day.