Edible Fungi
For the Volvariella volvacea V37 under the liquid culture industrialization production provides the concrete reference formula to the straw mushroom, and plants each kind of environmental condition request in the production process. The Volvariella volvacea V37 under the liquid culture conditions was studied, the different culture medium formula, the raise temperature, liquid volume in the bottled, the inoculation rate, pH and the culture time to its mycelium biomass were influenced. The results showed that: the optimal fermentation medium was formula B. Medium capacity 80 mL, inoculation rate 10%, pH was 6 to 9, the optimal culture conditions were temperature 30-32℃ and the culture time was from 6 days to 8 days.
with unique fragrances and a variety of health functions, Lentinula endodos is one of main cultivated mushroom in China. However, in some regions of China in recent years have witnessed varying degrees of mushroom virus disease,causing severe influences in yield and quality. Through experiences it showed that the virus is widely existed in Lentinula edodes.Three Lentinula edodes strains were selected to eliminate virus by picking up hypha tips(less than 0.2mm in length) . The results showed that:some strains with one band of dsRNA (11kb) left in Lentinula edodes strains were got, which called incomplete eliminated strain. only one strain was got with no dsRNA in it ,which called virus-free strain. the virus- elimination rate is very low by this method. The growth rate of the three kinds of strains in different pH were measured, it showed that the virus-free strain grew faster than virus-infected strain in pH 8 condition. cellulase, xylanase, laccase and polyphenol oxidase activity of the three kinds of strains were mensurated ,the result showed that the enzyme activity of Laccase and Polyphenol oxidase in virus-free strain were higher than that of virus-infected strain.Through the cultivation experiments ,the fruit body of virus-free strain is hypertrophy than others, and with a stronger anti-pollution ability.
Abstract : It was determined the virulence of Verticillium lecanii MZ041024 on Larvae and pupae of Lycoriella pleuroti. The bioassay results indicated that insecticidal activity of ethyl acetate was higher than that of others, their mortality rate of larvae and pupae respectively were 91.79% and 88.94%, with LC50 values of 1.745×105 spores /ml and 4.723×105 spores /ml ; LT50 was respectively3.51d and 3.78d, against Larvae and pupae of Lycoriella pleuroti , respectively.
Abstract: To investigate the characteristics and dynamic principle of the bile salt hydrolase produced by Kluyveromyces marxianus M3 from Tibetan Kefir, the single factor and multi levels orthogonal experiments was applied. Enzyme activity was tested under the different contitions:pH4~8, temperature31~43℃, concentration of substrate 4~8mmol/L, and different chemical reagents(SDS、EDTA、carbamide、Cu2+、Mg2+、Ca2+、Fe3+、Al3+、Mn2+ )during 30 min reaction between bile salt hydrolase and substrate. The optimal reaction condition of bile salt hydrolase was pH6.0, concentration of substrate 7mmol/L, temperature37℃. Metal ion of Mn2+、Fe3+、Ca2+ had comparatively large elevation effect on the enzyme activity, and other chemical reagents had few effect on the enzyme activity. The kinetic constant Km of bile salt hydrolase was 2.60 mmol/L. Strain M3 entered the stationary phases after 18~21h. The enzyme activity reached the maxlmum during log phase growth at18h. The experiments results showed the category of dynamic principle was growth couple synthesis type.
In order to select and breed new varieties of Lentinula edodes for export, the phylogenetic relationship of several strains of L. edodes was analyzed based on ribosomal DNA ITS sequences, and comparative experiments of cultivation between the two selected strains and some others cultivated in China were also investigated. The results indicated the moderate genetic diversity among these strains for export, and they could be clustered into two groups. The strains qh01, qh04, qh05 and qh06 were clustered into one group, which indicated a close phylogenetic relationship within the group. However, the single strain qh02 was clustered into another group, which inferred a far phylogenetic relationship with other strains. Comparative experiments showed various agronomic traits among the tested strains, and especially, the two strains qh02 and qh06, showed excellent agronomic traits, and consequently inferred that the two strains were suitable for export.
Edible Fungi
