小麦品系2004s-47的1Dx基因的克隆与序列分析

doi:10.11924/j.issn.1000-6850.casb15030014

中国农学通报 ›› 2015, Vol. 31 ›› Issue (21): 115-122.doi: 10.11924/j.issn.1000-6850.casb15030014

所属专题：生物技术；小麦

小麦品系2004s-47的1Dx基因的克隆与序列分析

相微微,王建武,刘宝龙,张怀刚

榆林学院生命科学学院,榆林学院生命科学学院,中国科学院西北高原生物研究所,中国科学院西北高原生物研究所

收稿日期:2015-03-03 修回日期:2015-06-30 接受日期:2015-04-24 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 相微微
基金资助:
榆林学院高层次人才科研启动基金项目 “榆林不同区域绿豆品种的加工品质特性研究” (14GK37)。

Molecular Cloning and Sequence Analysis of the 1Dx Gene in Wheat Line 2004s-47

Received:2015-03-03 Revised:2015-06-30 Accepted:2015-04-24 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 明确 2004s-47品系 1Dx基因的序列，为寻找新的优质 HMW-GS类型、实现小麦品质的改良及育种提供理论依据。应用 SDS-PAGE对 2004s-47品系中 HMW-GS的亚基组成进行分析，用 PCR技术克隆1Dx亚基基因并用 DNAMAN软件进行序列分析。结果从 2004s-47品系中扩增出了大小为 2514 bp的基因。该基因具有典型的小麦 HMW-GS x-型基因序列结构特征。又因为 1D比 1A和 1B基因组中的 x-型和 y-型基因易表达，结合 SDS-PAGE的结果，所以 2004s-47品系中 HMW-GS的亚基组成为(Null， 7+8， ?+10)。序列比对表明， 2004s-47品系的 1Dx？基因与节节麦(Aegilops tauschii) 1Dx2.1t (AF480486)同源性最高。将该序列提交到 NCBI，获得序列登陆号为： KP702118。2004s-47品系中 1Dx？序列的确定，为原核表达确定1Dx？是否为新的基因提供了基础，也为品质改良及育种提供了依据。

关键词: 西藏, 西藏, 冰雹日数, 时空分布, 变化规律

Abstract: Clarifying the sequence of 1Dx gene in 2004s-47 line would provide a theoretical basis for finding new types of high-quality HMW-GS and achieve wheat quality improvement and breeding. The high molecular weight glutenin subunits (HMW-GS) composition of 2004s-47 line was analyzed by SDS-PAGE. 1Dx subunit gene was cloned by PCR and sequence analysis was performed by DNAMAN. The results revealed that the gene sequence that cloned from 2004s-47 was 2514 bp and had typical x-type structure of wheat HMW-GS. And because the expressions of x- and y-type genes in the D genome of bread wheat were easier than that in the A and B genomes, together with the results of SDS-PAGE, the composition of 2004s-47 line was (Null， 7+ 8， ? + 10). Sequence alignments showed that the DNA sequence of the 1Dx？ gene from 2004s- 47 had the highest similarity to that of previously published 1Dx2.1t (AF480486) in Aegilops tauschii. The sequence was submitted to NCBI and sequence accession number: KP702118 was obtained. The identification of 1Dx gene sequence in 2004s-47 line provided a theoretical basis for the determination of whether 1Dx? was a new type of gene by prokaryotic expression, and for wheat quality improvement and breeding.

相微微,王建武,刘宝龙,张怀刚. 小麦品系2004s-47的1Dx基因的克隆与序列分析[J]. 中国农学通报, 2015, 31(21): 115-122.

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小麦品系2004s-47的1Dx基因的克隆与序列分析

Molecular Cloning and Sequence Analysis of the 1Dx Gene in Wheat Line 2004s-47

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