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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 85-92.doi: 10.11924/j.issn.1000-6850.2008-0774

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

DNA分子原位杂交在植物分子细胞遗传学研究中的应用

别同德,张伯桥,高德荣,程顺和,马谈斌   

  • 收稿日期:2008-08-28 修回日期:2008-09-09 出版日期:2008-11-05 发布日期:2008-11-05

Application of in situ Hybridization in Plant Molecular Cytogenetics Research

BIE Tong-De,derong gao   

  • Received:2008-08-28 Revised:2008-09-09 Published:2008-11-05 Online:2008-11-05

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摘要： DNA分子原位杂交（in situ hybridiation）是植物分子细胞遗传学研究的重要工具。本文简要回顾了DNA分子原位杂交的起源和发展，详细综述了基因组原位杂交（genomic in situ hybridization, GISH）在植物细胞遗传学研究中的应用以及荧光原位杂交（Fluorescence in situ hybridization, FISH）在物理作图和染色体识别中的应用。文中还介绍了Fiber-FISH、BAC-FISH以及Immuno-FISH等新兴技术。最后对FISH技术进行了展望。

关键词: 水稻, 水稻, 根癌农杆菌, 6-磷酸甘露糖异构酶基因, 雪花凝集素基因, 褐飞虱

Abstract: In situ hybridization is one of the most important techniques in plant molecular cytogenetics research. In this paper, we briefly introduced the origin and development of in situ hybridization, firstly. Then, application of genomic in situ hybridization (GISH) in plant cytogenetics research as well as fluorescence in situ hybridization (FISH) in plant physical mapping and chromosome identification were detailed reviewed. Some new techniques such as fiber-FISH, BAC-FISH, and immuno-FISH were also introduced in this paper. And a prospect about FISH was given out.