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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (13): 85-91.doi: 10.11924/j.issn.1000-6850.2011-3268

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

2株茶藨生柱锈重寄生木霉胞壁降解酶的基本性质研究

李靖 周利 陈玉惠 李永和   

  • 收稿日期:2011-11-07 修回日期:2012-02-17 出版日期:2012-05-05 发布日期:2012-05-05
  • 基金资助:

    云南省科技攻关项目“长防林重大病虫害华山松疱锈病、木蠹象监测和综合防治技术试验示范”;西南林业大学重点基金项目“一种抗五针松疱锈病生防菌剂研制及应用”

The Study on Degrading Enzymes of Cell Wall from Two Mycoparasites of Cronartium ribicola

  • Received:2011-11-07 Revised:2012-02-17 Online:2012-05-05 Published:2012-05-05

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781

摘要：

为了给β-1,3葡聚糖酶和几丁质酶的分离纯化及重寄生菌的利用提供理论依据，从茶藨生柱锈(Cronartium ribicola J.C.Fischer)的重寄生菌TR1和TR2中提取了β-1,3葡聚糖酶和几丁质酶，并进行了粗酶活性测定。结果显示，2菌株所产β-1,3-葡聚糖酶最适温度均为35℃，TR1所产β-1,3葡聚糖酶的最适pH 4.0，Km值为43.82 μg/mL，Ca2+是其激活剂，Cu2+、Fe3+、Al3+、Zn2+为其抑制剂。TR2所产β-1,3葡聚糖酶的最适pH5.0，Km为71.28 μg/mL，Ca2+和Mn2+是其激活剂，Cu2+、Fe3+、Al3+为其抑制剂。2菌株所产几丁质酶的激活剂为Mn2+和Ca2+，抑制剂为Cu2+。TR1所产几丁质酶的最适温度为40℃，最适pH6.0，Km为92.49 μg/mL；TR2所产几丁质酶的最适温度范围较宽为3~50℃，最适pH5.0，Km为71.11 μg/mL。TR1和TR2所产β-1,3-葡聚糖酶和几丁质酶稳定性较高，直接利用粗酶在野外防治疱锈病具有一定的可行性。

关键词: 油茶, 油茶, 间种, 土壤养分, 主成分分析

Abstract:

The aim was to provide the basis for the isolation and purification of β-1,3-glucanase and chitinase, and the use of mycoparasite. β-1,3-glucanase and chitinase were isolated from two mycoparasites (TR1 and TR2) of Cronartium ribicola. The enzymatic activities of β-1,3-glucanase and chitinase were detected. The results showed that optimum temperature of β-1,3-glucanase from TR1 and TR2 was 35℃. The optimum pH of β-1,3-glucanase from TR1 was 4.0 and Km was 43.82 μg/mL. Ca2+ was activator of the enzyme and Cu2+, Fe3+, Al3+, Zn2+ were inhibitors. The optimum pH of β-1,3-glucanase from TR2 was 5.0 and Km was 71.28 μg/mL. Ca2+ and Mn2+ were activators of the enzyme and Cu2+, Fe3+, Al3+ were inhibitors. Activators of chitinase from TR1 and TR2 were Mn2+ and Ca2+ and inhibitor was Cu2+. The optimum temperature of chitinase from TR1 was 40℃ and the optimum pH was 6.0, Km of the enzyme was 92.49 μg/mL. The optimum temperature of chitinase from TR2 was from 30℃ to 50℃. The optimum pH was 5.0 and Km was 71.11 μg/mL. β-1,3-glucanase and chitinase produced by TR1 and TR2 possess high stability, so that it was feasible to use crude enzyme directly in field control of blister rust.