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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 254-258.doi: 10.11924/j.issn.1000-6850.2011-3692

所属专题： 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

烟草青枯菌特异性引物筛选及其应用

杨姣弟 陈武 王运生 肖启明   

  • 收稿日期:2011-12-07 修回日期:2012-02-04 出版日期:2012-04-05 发布日期:2012-04-05

Screening and Application of Ralstonia solanacearum Specific-primers

  • Received:2011-12-07 Revised:2012-02-04 Online:2012-04-05 Published:2012-04-05

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摘要：

为解决快速诊断烟田土壤中是否含有烟草青枯菌这一问题，基于已公布的5个青枯菌基因组全基因组比对结果，设计9对青枯菌特异性引物，筛选并验证得到3对引物能对青枯菌进行特异性扩增。应用筛选得到的引物能对土壤中烟草青枯菌进行特异性检测，初步认为本方法对土壤青枯菌最低检出率为2×106/g，为土壤中快速检测烟草青枯菌提供了快速、准确诊断的新方法。

关键词: 相关性, 相关性

Abstract:

For the method of rapid diagnosis of Ralstonia solanacearum contain in tobacco fields, this paper based on the comparison result of the five whole-genome of the published genomes of Ralstonia solanacearum, designed nine pairs of specific primers of Ralstonia solanacearum, and has achieved three pairs of primers for specific amplification of Pseudomonas solanacearum by screening and verification. The application of the screened primers could be used as an effective method to do specific detection of tobacco Ralstonia solanacearum in the soil. As a preliminary conclusion, the lowest detection rate of Ralstonia solanacearum in the soil was 2×106/g. Thus, it’s a rapid and accurate new way for detection of tobacco Ralstonia solanacearum in the soil.