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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (18): 53-58.doi: 10.11924/j.issn.1000-6850.2012-1038

• 农学 农业基础科学 • 上一篇    下一篇

快中子诱变小豆京农6号突变体筛选

张李 赵波 万平 骆晚侠 李奕松 杨凯 曹劲宏 孙祎振 赵翠格 张海明   

  • 收稿日期:2012-03-22 修回日期:2012-04-24 出版日期:2012-06-25 发布日期:2012-06-25
  • 基金资助:

    北京市教委科研计划面上项，北京市教委2011科基-科创平台项目

The Screening of Mutants from Jingnong 6 Induced by Fast Neutron in Azuki Bean (Vigna angularisi)

  • Received:2012-03-22 Revised:2012-04-24 Online:2012-06-25 Published:2012-06-25

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摘要：

为了进行小豆种质创新和获得基因定位和克隆及其基因功能分析的突变体材料，分别用30、35、40、45、50、55 Gy剂量的快中子，辐照小豆‘京农6号’干种子。M1出苗率分别为31.72%、29.06%、30.29%、36.51%、16.15%和15.17%，变异率为3.20%、4.70%、6.75%、6.61%、4.00%和2.20%。辐射剂量增加，出苗率降低、变异率呈增加趋势，M3代共获得77份叶形、株型、粒型、籽粒大小、粒色、叶色、早熟、丰产等性状变异材料。40~45 Gy为小豆快中子诱变的适宜剂量，这些突变体为小豆相关基因定位克隆、功能分析和育种提供了有价值的材料。

关键词: 理化性质, 理化性质

Abstract:

The aim was to obtain the mutant materials of azuki bean for gene location, clone and gene functional analysis. Dry seeds of azuki bean variety ‘Jingnong 6’ were irradiated with 30, 35, 40, 45, 50, 55 Gy dosage fast neutron respectively for creating new germplasm and obtaining mutants of gene mapping, cloning and functional analysis. Germination rates of M1 generation were 31.72%, 29.06%, 30.29%, 36.51%, 16.15% and 15.17% respectively. Their relative variation frequencies were 3.20%, 4.70%, 6.75%, 6.61%, 4.00%, 2.20%. The higher dosage of fast neutrons was, the lower germination rate was, and the higher variation frequence was relative. The dosages from 40 to 45 Gy were optimistic to azuki bean fast neutron mutagenesis. Seventy seven mutants including leaf shape, plant architecture, seed shape and size, seed coat color, leaf color, early maturity and high yield in M3 generation were gained. These mutants were very useful for gene mapping, gene cloning, further functional analysis and breeding.

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