高通量测序技术在土壤微生物多样性研究中的应用

doi:10.11924/j.issn.1000-6850.2013-2513

摘要/Abstract

摘要： 为了阐明高通量测序技术在土壤微生物多样性研究中的应用前景，归纳介绍了Solexa、454和Ion Torrent等常用高通量测序技术的原理和优点，综述了近年高通量测序技术在土壤微生物物种多样性、结构多样性、功能多样性和遗传多样性四个方面应用的研究进展，总结了高通量测序技术应用中存在的问题，并分析展望了其在土壤微生物多样性研究中的发展趋势。

关键词: 稻瘟病, 稻瘟病, 化学防治, 抗性品种, 生物防治, 栽培措施

Abstract: In order to illuminate the application prospects of high-throughput sequencing techniques in soil microbial diversity, this article gave a summarization and introduction of principles and advantages of common high-throughput sequencing techniques, including Solexa, 454 and Ion Torrent. The latest research advances of high- throughput sequencing techniques applications were reviewed in soil microbial species diversity, structures diversity, functions diversity and genes diversity. Finally, the application problems and the development tendency of high- throughput sequencing techniques used in soil microbial studies were summarized and discussed.

楼骏柳勇李延. 高通量测序技术在土壤微生物多样性研究中的应用[J]. 中国农学通报, 2014, 30(15): 256-260.

Li Yan. Review of High-throughput Sequencing Techniques in Studies of Soil Microbial Diversity[J]. Chinese Agricultural Science Bulletin, 2014, 30(15): 256-260.

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