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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 23-29.doi: 10.11924/j.issn.1000-6850.casb14120015

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

腐胺对白桦悬浮细胞中激素和黄酮积累影响的初步研究

周文洋,孙菲菲,梁佳,詹亚光,范桂枝   

  1. 东北林业大学S生命科学学院,东北林业大学S生命科学学院,东北林业大学S生命科学学院,东北林业大学S生命科学学院,东北林业大学S生命科学学院
  • 收稿日期:2014-12-02 修回日期:2015-01-11 接受日期:2015-01-23 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 范桂枝
  • 基金资助:
    国家自然基金项目“PAs在真菌诱导白桦酯醇合成中的作用机理研究”(31100445)；中央高校基本科研业务专项资金项目“信号分子对药用植物代谢物合成的调控作用研究”(DL13EA08-03)；黑龙江省博士后科研启动金“PAs和H2O2在真菌促进白桦三萜合成中的cross-talk研究”(415288)。

Effect of Putrescine on Hormone and Flavonoid Accumulation in Betula platyphylla Suspension Cultured Cells

  • Received:2014-12-02 Revised:2015-01-11 Accepted:2015-01-23 Online:2015-06-02 Published:2015-06-02

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摘要： 为明确腐胺对白桦悬浮细胞中激素和黄酮累积的影响，在白桦悬浮细胞生长的第8 天添加不同浓度的腐胺、脱落酸(ABA)、茉莉酸甲酯(MeJA)、生长素(IAA)和玉米素核苷(ZR)，采用紫外分光光度计法分析白桦悬浮细胞中黄酮含量和利用酶联免疫方法测定ABA、JA、IAA和ZR含量。外源腐胺和激素的添加均不同程度地提高了白桦悬浮细胞中黄酮的累积量，其中1 mmol/L 腐胺处理24 h 时增幅最高，增加了97.90%；激素中1 mg/LABA处理后增幅最高，增加了103.40%。腐胺和激素共同处理后黄酮含量的增幅均较其单独处理提高。其中1 mg/L 腐胺与5 mg/L JA 处理后黄酮含量增幅最高，比对照和腐胺处理分别增加了197.43%和103.57%。同时腐胺处理后增加了IAA 和ZR的含量，而降低了ABA和JA 含量。进一步分析其相关性发现，腐胺处理后激素、激素比与黄酮含量的相关性增幅不同，其中JA与黄酮含量的相关性增幅最大，由对照的-0.883 变为0.903。腐胺与ABA、JA、IAA和ZR协同促进了白桦悬浮细胞中黄酮的累积。

关键词: 农产品, 农产品, 膳食暴露评估, 模型构建

Abstract: The aim was to analyze the effect of exogenous putrescine on hormone and flavonoid accumulation in Betula platyphylla suspension cultured cells. Various concentrations of putrescine and exogenous hormone were added to the eight-day-old suspension cultured cells, the content of flavonoid, ABA, JA, IAA and ZR were analyzed by UV spectrophotometer and ELISA. Both exogenous putrescine and hormone treatment increased the content of flavonoid in birch suspension cultured cells. Among them, the highest flavonoid content was induced by 1 mmol/L putrescine for 24 h, the increasing rate was 97.90% . Among the hormone treatment, the highest flavonoid content was induced by 1 mg ABA induction for 24 h, the increasing rate was 103.40%. The joint treatment of putrescine and hormone increased much more flavonoid content than that of control or putrescine treatment. The highest flavonoid content was induced by 1 mmol/L putrescine and 5 mg/L JA, the increasing rate was 197.43% and 103.57% compared with control and putrescine treatment, respectively. At the same time, putrescine increased the content of IAA and ZR, but decreased the content of ABA and JA. To further analyze the correlation, we found the correlation between hormones or hormone ratio with flavonoid content was different, JA treatment showed the highest change from -0.883 to 0.903. It can be inferred that the interaction between putrescine and hormone (ABA, JA, IAA and ZR) regulated the flavonoid production in Betula platyphylla suspension cultured cells.