SPR生物传感技术在农产品安全检测中的应用

doi:10.11924/j.issn.1000-6850.casb18110039

中国农学通报 ›› 2020, Vol. 36 ›› Issue (6): 93-99.doi: 10.11924/j.issn.1000-6850.casb18110039

SPR生物传感技术在农产品安全检测中的应用

王婷婷¹^,², 胡建东²()

¹ 河南农大迅捷测试技术有限公司,郑州 450002
² 河南农业大学机电工程学院,郑州 450002

收稿日期:2018-11-12 修回日期:2019-01-18 出版日期:2020-02-25 发布日期:2020-02-22
通讯作者: 胡建东
作者简介:王婷婷,女,1984年出生,河南商丘人,中级,硕士,主要从事SPR技术分析测试方法研究。通信地址：450002 河南农大迅捷测试技术有限公司,Tel：0371-55611860,E-mail：ting.moon.w@163.com。
基金资助:
国家重点研发计划“土壤生物有效态氮磷原位提取及检测设备研发与标准研制”(2017YFD0801204);河南省自然科学基金“植物胁迫激素脱落酸光谱检测新技术研究”(162300410143);郑州市第三批“智慧郑州·1125聚才计划”项目“便携式金标判读仪的研究与开发”(郑政[2018]45号);郑州市工程技术研究中心“郑州市农业检测工程技术研究中心”(郑科[2017]91号)

Applications of SPR Biosensing Technology in Agricultural Products Safety Detection

Wang Tingting¹^,², Hu Jiandong²()

¹ Henan Nongda Xunjie Measurement Technology Co., Ltd, Zhengzhou 450002
² Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002

Received:2018-11-12 Revised:2019-01-18 Online:2020-02-25 Published:2020-02-22
Contact: Hu Jiandong

摘要/Abstract

摘要：

表面等离子共振作为近几年在国内发展起来的一种检测技术,具备检测样品用量少、灵敏度高、检测速度快等优点,已被用于环境检测、医疗诊断、药物筛选、蛋白质组学、基因组学等诸多领域。随着技术的发展,广大学者将其与生物技术结合起来,在传感芯片表面修饰不同的特异性抗原抗体及信号放大因子,将表面等离子共振技术不断应用到农产品安全的检测中。通过介绍该技术在农产品中农残药残、动物疾病、微生物、食品添加剂、重金属及转基因检测的应用情况,以期使读者深入了解表面等离子共振技术在食品安全检测的发展现状,为其在该领域的进一步发展提供可以借鉴的资料。

关键词: 表面等离子共振, 生物传感, 农产品安全, 检测, 应用

Abstract:

Surface plasmon resonance (SPR) as a novel technology has advantages of small sample volumes, high sensitivity and fast detection speed, which is widely applied in environmental detection, medical diagnosis, drug screening, proteomics, genomics and many other fields. With its development, SPR is combined with biotechnology, then modified by specific antigen, antibody and signal amplification factor, and widely used in safety detection of agricultural products. The paper introduces the application of SPR in detection of pesticide and medicine residues, animal disease, microbe, food additives, heavy metal and transgenic test in agricultural products. The study could provide certain references for understanding the development status of SPR in food safety test.

Key words: surface plasmon resonance, biosensing, agricultural products safety, detection, application

中图分类号:

R155.5

王婷婷, 胡建东. SPR生物传感技术在农产品安全检测中的应用[J]. 中国农学通报, 2020, 36(6): 93-99.

Wang Tingting, Hu Jiandong. Applications of SPR Biosensing Technology in Agricultural Products Safety Detection[J]. Chinese Agricultural Science Bulletin, 2020, 36(6): 93-99.

图/表 6

图1. SPR检测原理

图2. SPR检测盐酸克伦特罗的响应过程[17]

图3. a检测猪圆环病毒Ⅱ型的自组装分子识别膜;b不同浓度病毒的检测响应曲线[29] a~g的PCV2浓度分别为10.0、7.5、5.0、2.5、1.0、0.5 mg/mL

图4. a检测辣椒粉和腌制品中的苏丹红Ⅰ的SPR传感芯片光谱图;b检测辣椒粉和腌制品中的苏丹红Ⅰ的Au、NIP、MIP及MUA/Au的阻抗图谱[32]

图5. a不同浓度的氯化钠溶液在金银复合膜的传感图;b检测不同折射率的氯化钠溶液的计算曲线[34]

图6. a不同浓度转基因片段的SPR响应曲线;b不同浓度转基因片段的PCR电泳结果[37] M. DNA marker;1~5样品质量浓度分别为11.5、7.1、3.9、1.9、1.5 mg/L;6.空白对照

参考文献 42

[1]	徐霞, 成芳, 应义斌 . 近红外光谱技术在肉品检测中的应用和研究进展[J]. 光谱学与光谱分析, 2009,29(7):1876-1880.
[2]	傅若农 . 近几年国内外气相色谱固定相的进展[J]. 分析试验室, 2013,32(5):111-124.
[3]	Wang Q J, Yi R H, Mo C H . Determination of Quinolone Antibiotics in Water Using Solid Phase Extraction-High Performance Liquid Chromatography-Fluorescence Method[J]. Animal Husbandry and Feed Science, 2011,3(2):45-48.
[4]	Choma I, Grenda D, Malinowska I , et al. Determination of flumequine and doxycycline in milk by a simple thin-layer chromatographic method[J]. JChromatogr B, 1999,734(1):7-14.
[5]	王磊, 张丽杰, 吕伟 , 等. 生物素-亲和素放大酶联免疫吸附法测定二乙基磷酸酯类有机磷农药[J]. 分析化学, 2011,39(3):346-350.
[6]	杨丽 . 食品污染物检测技术研究进展与食品安全检测方法标准研究[J]. 中国食物与营养, 2005,3:31-33.
[7]	Homola J, Yee S S, Gauglitz G . Surface plasmon resonance sensors:review[J]. Sensors and Actuators B:Chemical, 1999,54(1-2):3-15.
[8]	Rasooly A . Surface Plasmon Resonance Analysis of Staphylococcal Enterotoxin B in Food[J]. Journal of Food Protection, 2001,64(1):37-43.
[9]	Mifumi S, Yoko N, Zhang W , et al. Simple and rapid detection method using surface plasmon resonance for dioxins, polychlorinated biphenylx and atrazine[J]. Analytica Chimica Acta, 2001,434(2):223-230.
[10]	黄智伟, 黄琛 . 表面等离子体共振生物传感器的研究现状[J]. 传感器世界, 2001,7(5):8-12.
[11]	Liedberg B, Nylander C, Lundstrom I . Surface plasmon resonance or gas detectlon and biosensing[J]. Sensors &Actuators, 1983,4:299-304.
[12]	Inamori K, Kyo M, Nishiya Y , et al. Detection and quantification of on-chip phosphorylated peptides by surface plasmon resonance imaging techniques using a phosphate capture molecule[J]. Analytical Chemistry, 2005,77(13):3979-3985.
[13]	Chen C Y, Baker S, Darton R . The application of a high throughput analysis method for the screening of potential biosurfactants from natural sources[J]. Journal of Microbiological Methods, 2007,70(3):503-511.
[14]	Altintas Z, Uludag Y, Gurbuz Y , et al. Development of surface chemistry for surface plasmon resonance based sensors for the detection of proteins and DNA molecules[J]. Analytica Chimica Acta, 2012,7(12):138-144.
[15]	Liu X . Performance Improvement for the Wavelength Modulation Surface Plasmon Resonance and Its Application[D]. Changchun: Jilin University, 2006.
[16]	鲍蕾, 许艳丽, 梁成珠 , 等. 表面等离子共振生物传感器在食品检测中的应用[J]. 检验检疫学刊, 2012,22(4):73-76.
[17]	Hu J D, Chen R P, Wang S , et al. Detection of Clenbuterol Hydrochloride Residuals in Pork Liver Using a Customized Surface Plasmon Resonance Bioanalyzer[J/OL]. PLOS ONE, 2015,10(3):e0122005.
[18]	石婷, 刘瑾, 张婉洁 , 等. 基于SPR生物传感器的抗生素残留检测及影响因素分析[J]. 天津大学学报, 2010,43(3) 255-261.
[19]	Edward L, Fafara A, Vandernoot V , et al. Surface plasmon resonance sensors using molecularly imprinted polymers for sorbent assay of theophylline, caffeine, and xanthine[J]. Canadian Journal of Chemistry, 1998,76(3):265-273.
[20]	Anand S, Usha S, Gupta B . Fiber optic profenofos sensor based on surface plasmon resonance technique and molecular imprinting[J]. Biosensors and Bioelectronics, 2016,5(79):150-157.
[21]	Zhang L L, Zhu C C, Chen C B , et al. Determination of kanamycin using a molecularly imprinted SPR sensor[J]. Food Chemistry, 2018,266(15):170-174.
[22]	LonL A, Musiek M. D, Natan M. J . Colloidal Au-Enhanced surface plasmon resonance immunosensing[J]. Analytical Chemistry, 1998,70(24):5177-5183.
[23]	Lee W, Son T, Lee C , et al. Ultra-Sensitive Surface Plasmon Resonance Detection by Colocalized 3D Plasmonic Nanogap Arrays[J]. Biosensors and Biodetection, 2017,3:15-29.
[24]	Lee S J, Youn B S, Park J W , et al. SSDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes[J]. Analytical Chemistry, 2008,80(8):2867-2873.
[25]	Bianco M, Sonato A, De Girolamo A , et al. An aptamer-based SPR-polarization platform for high sensitive OTA detection[J]. Sensors and Actuators B: Chemical, 2017,3(241):314-320.
[26]	Wang S, Dong Y Y, Liang X G . Development of a SPR aptasensor containing oriented aptamer for direct capture and detection of tetracycline in multiple honey samples[J]. Biosensors and Bioelectronics, 2018,109:1-7.
[27]	邵康, 聂阿秀, 韩鹤友 . 动物重大疾病的光电学传感技术新进展[J]. 分析科学学报, 2017,33(5):729-735.
[28]	Hu J D, Li W, Wang T T , et al. Development of a label-free and innovative approach based on surface plasmon resonance biosensor for on-site detection of infectious bursal disease virus (IBDV)[J]. Biosensors and Bioelectronics, 2012,31(1):475-479.
[29]	Hu J D, Wang T T, Wang S , et al. Development of a surface plasmon resonance biosensing approach for the rapid detection of Porcine Circovirus Type2 in sample solutions[J/OL]. PLOS ONE, 2014,9(10):e111292.
[30]	Wang Y X, Ye Z Z, Si C Y , et al. Subtractive Inhibition Assay for the Detection of E.coli O157:H7 Using Surface Plasmon Resonance[J]. Sensors, 2013,11(3):2728-39.
[31]	Naboka A, Al-Rubaye A G, Al-Jawdah A M , et al. Novel optical biosensing technologies for detection of Mycotoxins[J]. Optics and Laser Technology, 2019,109:212-221.
[32]	周春燕, 陈长宝, 李洁 , 等. 苏丹红Ⅰ印迹表面等离子共振传感器的制备与表征[J]. 应用化学, 2014,31(19):1107-1114.
[33]	王曼丽, 明华蜜, 尹洪宗 , 等. 表面等离子体共振生物传感器的构建及对柠檬黄的检测[J]. 分析化学研究报告, 2014,42(1):53-58.
[34]	Pang K, Dong W, Zhang B , et al. A Performance-Enhanced Bimetallic Chip for the Detection of Cadmium Ions with Surface Plasmon Resonance[J]. Plasmonics, 2016,11(4):1119-1128.
[35]	Pelossof G, Telvered R, Willner I . Amplified surface plasmon resonance and electrochemical detection of Pb ²⁺ ions using the Pb ²⁺-dependent DNAzyme and hemin/G-quadruplex as a label [J]. Analytical Chemisry, 2012,84(8):3703-3709.
[36]	肖守斌 . 运用表面等离子共振(SPR)生物传感器检测转基因玉米的研究[J]. 玉米科学, 2009,17(2):38-43.
[37]	王婷婷, 李伟, 魏文松 , 等. 光学表面等离子共振生物传感器检测小麦转基因的研究[J]. 河南农业大学学报, 2013,47(5):580-583.
[38]	Nasrin F, Chowdhury A D, Takemura K , et al. Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots[J]. Biosens Bioelectron, 2018,30(122):16-24.
[39]	Chiu N F, Yang C D, Chen C C , et al. Stepwise control of reduction of graphene oxide and quantitative real-time evaluation of residual oxygen content using EC-SPR for a label-free electrochemical immunosensor[J]. Sensors and Actuators B, 2018,258:981-990.
[40]	Otipka P, Vlče J, Leňák M , et al. Design of MO-SPR sensor element with photonic crystal[J]. Photonics and Nanostructures-Fundamentals and Applications, 2018,31:77-80.
[41]	Liu H, Wang M, Wang Q , et al. Simultaneous measurement of hydrogen and methane based on PCF-SPRstructure with compound ﬁlm-coated side-holes[J]. Optical Fiber Technology, 2018,45:1-7.
[42]	Meyer Stefan A, Auguié B, Le Ru Eric C , et al. Combined SPR and SERS microscopy in the Kretschmann configuration[J]. Journal of Physics, 2012,116(3):1000-1007.

SPR生物传感技术在农产品安全检测中的应用

Applications of SPR Biosensing Technology in Agricultural Products Safety Detection

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 42

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	曾端香, 余曦玥, 于敬文, 贾建平, 彭德良, 黄文坤. 松材线虫病的检测及综合防治技术[J]. 中国农学通报, 2022, 38(4): 86-91.
[2]	强生军, 刘玉荣, 李刚. 溶剂标、基质标对农药残留检测结果的影响及校正[J]. 中国农学通报, 2022, 38(4): 99-106.
[3]	番华彩, 曾莉, 李卫雁, 丁云秀, 郭志祥, 李舒, 徐胜涛, 郑泗军, 王永斌. 香蕉细菌性软腐病防控药剂筛选及田间应用效果评价[J]. 中国农学通报, 2022, 38(31): 113-118.
[4]	陈丹丹, 万建春, 连琦, 王建雄, 王栋, 洪挺, 杨毅生. 中药材农药残留研究进展[J]. 中国农学通报, 2022, 38(31): 125-135.
[5]	唐玲, 孙思琦, 闫桦, 刘亚昕, 刘大丽. 甜菜红素提取与纯化技术的研究进展[J]. 中国农学通报, 2022, 38(28): 136-142.
[6]	骆美, 郭龙, 费坤, 张天恩, 李陈, 马友华. 耕地质量提升技术及其应用[J]. 中国农学通报, 2022, 38(21): 76-81.
[7]	陈培华, 鲍涵, 陈培寿, 沈万宽. 果蔗健康种苗田间效果评价[J]. 中国农学通报, 2022, 38(16): 1-5.
[8]	李文业, 徐放, 王海华, 朱报著. ‘广东含笑’研究进展[J]. 中国农学通报, 2022, 38(14): 47-52.
[9]	刘亚昕, 闫桦, 唐玲, 谭文勃, 兴旺, 刘大丽. 红甜菜和甜菜红素的综合应用研究进展[J]. 中国农学通报, 2022, 38(13): 157-164.
[10]	陈芳玲, 樊娅萍, 贺苗苗, 王倡宪. 丛枝菌根真菌在药用植物上的应用研究进展[J]. 中国农学通报, 2022, 38(12): 55-60.
[11]	昝光敏, 张玲, 张延瑞, 盛英华, 周凯, 王贤智. 大豆籽粒脂肪酸组分气相色谱检测方法的建立[J]. 中国农学通报, 2021, 37(9): 118-124.
[12]	李玮. 新型除草剂氟咯草酮在青海高原马铃薯田的应用及安全性[J]. 中国农学通报, 2021, 37(9): 149-154.
[13]	文英, 刘永生. 基于ipaH基因的志贺菌LAMP检测方法的建立及应用[J]. 中国农学通报, 2021, 37(5): 104-110.
[14]	李楠楠, 李顺成, 韩玉林, 邹少奎, 杜晓宇, 杨光宇, 王丽娜, 张倩, 吕永军. Fhb1基因改良‘周麦’品种赤霉病抗性研究[J]. 中国农学通报, 2021, 37(4): 98-104.
[15]	王东鹏, 叶诚, 廖小丽. 微流控芯片在农产品安全检测中的应用[J]. 中国农学通报, 2021, 37(36): 148-154.