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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (23): 47-52.doi: 10.11924/j.issn.1000-6850.casb15030235

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

共培养提高解磷菌解无机磷能力及解无机磷基因(pqqE)的克隆

李兴霖,秦平,葛菁萍,平文祥   

  1. 黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院,黑龙江大学生命科学学院
  • 收稿日期:2015-03-27 修回日期:2015-05-10 接受日期:2015-06-08 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 平文祥
  • 基金资助:
    国家自然科学基金“表达IBDV主要保护性抗原的重组杆状病毒构建及其作为疫苗效果研究”(31270143)；国家自然科学基金“菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制”(31270534)；国家自然科学基金“副干酪乳杆菌与枯草芽孢杆菌等菌群种间关系以及协同合作策略的研究”(31470537)；国家自然科学基金青年科学基金项目“酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制”(31300355)；黑龙江省高等学校科技创新团队“农业微生物发酵技术”(2012td009)。

Enhancement Effect of Co-culture on the Phosphate Solubilizing Ability of Phosphate Solubilizing Bacteria and the Clone of Phosphate Solubilizing Gene pqqE

  • Received:2015-03-27 Revised:2015-05-10 Accepted:2015-06-08 Online:2015-08-20 Published:2015-08-20

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摘要： 在土壤磷循环相关的生态学系统中，解磷微生物担任着重要的角色，它能将难溶性磷转化为可溶性磷，从而提高磷肥的利用率。通过磷钼蓝比色法对9株解磷菌的解磷能力进行检测，从中选取3株解磷能力较强的菌株并按不同的接种量比例两两共培养，测定共培养后发酵液中可溶性磷含量并比较解磷能力。结果表明，在共培养过程中，当将苏云金芽胞杆菌HDBP2与蜡状芽胞杆菌HDBP5以2:1比例混合培养时，混合物解磷能力最强，培养液中可溶性磷含量最高可达到90.25μg/mL。为了进一步了解解磷菌解无机磷的能力，同时对苏云金芽胞杆菌HDBP2、蜡状芽胞杆菌HDBP4和HDBP5中可促进细菌溶解利用无机磷的吡咯喹啉醌合成基因(pqqE)进行克隆，进而从分子生物学角度阐明解磷菌解无机磷的机理。苏云金芽胞杆菌HDBP2与蜡状芽胞杆菌HDBP5共培养有助于提高菌株解磷能力。

关键词: 农地边际化, 农地边际化, 土地利用, 诊断标准, 边际收益

Abstract: Phosphate solubilizing microorganisms (PSM) play important roles in the phosphorus cycle, which could transform insoluble phosphate into soluble phosphate and increase the availability of phosphate fertilizer. In this study, the phosphate solubilizing ability of 9 phosphate solubilizing microorganisms were detected by the method of phosphomolybdate-blue spectrophotometry and 3 of them were selected with better phosphate solubilizing ability, then every 2 of this 3 strains were co-cultivated in different inoculation proportions, the contents of dissoluble phosphorus in liquid cultures were determined and the phosphate solubilizing abilities were compared. The results indicated that the best phosphate solubilizing ability during the fermentation process was obtained in the culture medium when B.thuringiensis HDBP2 and B.cereus HDBP5 were cocultivated at the ratio of 2:1, and the soluble phosphorous content could reach 90.25 μg/mL. Furthermore, the pqqE gene of B.thuringiensis HDBP2, B.cereus HDBP4 and HDBP5 which could promote the dissolution was cloned to further illuminate the phosphate solubilizing mechanism in molecule biology. The co-culture of B. thuringiensis HDBP2 with B.cereus HDBP4 could enhance their phosphate solubilizing abilities.