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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (30): 225-230.doi: 10.11924/j.issn.1000-6850.casb15040054

所属专题： 马铃薯

• 生物技术科学 • 上一篇    下一篇

SRAP和SSR标记对马铃薯遗传多样性的差异分析

甘晓燕,程永芳,巩 檑,宋玉霞   

  1. (宁夏农业生物技术重点实验室,银川 750002）
  • 收稿日期:2015-04-07 修回日期:2015-05-15 接受日期:2015-05-22 出版日期:2015-10-29 发布日期:2015-10-29
  • 通讯作者: 甘晓燕
  • 基金资助:
    本研究由现代农业产业技术体系专项基金(CARS-10-ES30)；宁夏自然科学基金项目“宁夏马铃薯抗旱机制的转录组分析及关键基因功能验证”(NZ13098)，“抗旱转录因子NAC遗传转化马铃薯的研究”(NZ14210)；宁夏农林科学院科技创新先导资金项目“宁夏马铃薯抗旱机制及功能基因研究”(NKYJ-13-05)，“马铃薯生物技术育种研究”(NKYG-14-05)。

Variance Analysis of Genetic Diversity of Potato with SRAP and SSR Markers

Gan Xiaoyan, Cheng Yongfang, Gong Lei, Song Yuxia   

  1. (Key Lab of Agricultural Biotechnology, Yinchuan 750002)
  • Received:2015-04-07 Revised:2015-05-15 Accepted:2015-05-22 Online:2015-10-29 Published:2015-10-29

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摘要： 掌握宁夏地区主要马铃薯品种的遗传多样性，了解其遗传背景，明确各品种间的亲缘关系为马铃薯育种提供理论依据。利用SSR和SRAP 2种分子标记对47份马铃薯种质材料进行遗传多样性分析，并对2种分子标记结果进行比较。12对多态性SRAP标记共检测到180个多态性位点，平均每对引物检测到15个多态性位点，每个SRAP位点的PIC值为0.2~0.43，平均值为0.34；47份马铃薯种质资源遗传相似性系数为0.57~0.83。15对多态性SSR标记检测到80条多态性位点，平均每对引物检测到 5.3个多态性位点，每个SSR位点的PIC值为0.37~0.72，平均值为0.51；马铃薯种质材料遗传相似性系数为0.60~0.97。根据系谱资料分析发现，SRAP标记更适用于遗传关系较近材料的遗传多样性分析。宁夏地区主要的马铃薯品种遗传相似度较低，亲缘关系较远。

关键词: 猕猴桃, 猕猴桃, 生产, 贸易, 竞争力, 建议

Abstract: This study was to understand the genetic diversity and genetic background of the main potato varieties in Ningxia, clarify the relationship among varieties, and provide the theoretical basis for potato breeding. The genetic diversity of 47 potato germplasm materials was studied with SSR and SRAP markers, and the results of these two maker systems were compared. The results showed that 180 polymorphic fragments were detected using 12 SRAP primer pairs, with an average number of polymorphic fragments per primer pair of 15. The PIC for SRAPs was from 0.2 to 0.43 with an average of 0.34. The genetic similarity coefficient of the 47 germplasm materials varied from 0.57 to 0.83 by SRAP markers. Whereas, 80 polymorphic fragments were detected using 15 SSR primer pairs, with an average number of polymorphic fragments per primer pair of 5.3. The PIC for SSRs was from 0.37 to 0.72 with an average of 0.51. The genetic similarity coefficient of the 47 germplasm materials varied from 0.60 to 0.97 by SSR markers. According to the pedigree information, the SRAP markers were more effective for genetic analysis of closely related lines. Potato varieties in Ningxia had low genetic similarity and distant genetic relationship.