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中国农学通报 ›› 2015, Vol. 31 ›› Issue (34): 197-202.doi: 10.11924/j.issn.1000-6850.casb15050005

所属专题: 生物技术 园艺

• 植物保护 农药 • 上一篇    下一篇

草莓新品种‘晶玉’抗炭疽病相关的生理生化指标分析

张 鹏1,2,曾祥国1,韩永超1,向发云1,顾玉成1   

  1. (1湖北省农业科学院经济作物研究所,武汉 430064;2中南民族大学生命科学学院,武汉 430074)
  • 收稿日期:2015-05-04 修回日期:2015-11-19 接受日期:2015-06-23 出版日期:2015-12-17 发布日期:2015-12-17
  • 通讯作者: 张鹏
  • 基金资助:
    国家农业科技成果转化资金项目“抗病高产草莓新品种‘晶玉’中试与示范”(2012GB2D100298);农业部公益性行业科研专项项目“南方地区草莓品种的选育及优质高效栽培关键技术研究”(201003064);湖北省农业科技创新中心资助项目“草莓、魔芋高效安全生产关键技术研究与应用”(2007-620-006-003);湖北省农业科学院青年科学基金“草莓新品种‘晶玉’的抗炭疽病机理研究”(2012NKYJJ05)。

Study on Physiological and Biochemical Indexes in the Resistance of ‘Jingyu’ to Strawberry Anthracnose

Zhang Peng1,2, Zeng Xiangguo 1, Han Yongchao1, Xiang Fayun1, Gu Yucheng1   

  1. (1Institute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064;2College of Life Science, South-Central University for Nationalities, Wuhan 430074)
  • Received:2015-05-04 Revised:2015-11-19 Accepted:2015-06-23 Online:2015-12-17 Published:2015-12-17

摘要: 为了研究草莓新品种‘晶玉’抗炭疽病特性的生理生化机制,以‘晶玉’为研究对象,高抗炭疽病品种‘甜查理’和感病品种‘丰香’、‘红颜’为对照,分别检测接种炭疽菌后不同时间点的可溶性总蛋白、丙二醛(MDA)、维生素C含量,以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活力的变化。结果表明:在96 h检测范围内,6个生理生化指标的变化趋势总体表现为先升高后降低;抗、感品种间可溶性总蛋白、MDA含量以及SOD、POD活力差异显著;MDA含量随品种抗炭疽病能力增强而降低,CAT活力随品种抗炭疽病能力增强而增加。研究结果说明,‘晶玉’抗炭疽病特性与其低MDA含量、高的可溶性蛋白含量,以及高的SOD、POD、CAT活力相关。

关键词: 红花檵木, 红花檵木, 离体叶片, 再生

Abstract: In order to research the physiological and biochemical mechanisms of the resistance of a new strawberry cultivar ‘Jingyu’ to anthracnose, we used ‘Jingyu’ as a research object and the high-anthracnose-resistant strawberry cultivar ‘Sweet Charlie’, anthracnose-susceptible strawberry cultivars ‘Toyonoka’ and ‘Benihoppe’ as controls to investigate the changes of the content of total soluble proteins, malondialdehyde (MDA) and vitamin C, as well as the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the leaves of the four strawberry cultivars at different times after inoculated with Colletotrichum gloeosporioides. The results showed that the content of total soluble proteins, MDA and vitamin C and the activity of SOD, POD, CAT in the leaves increased firstly and then decreased in a 96 hours range. The content of soluble total protein and MDA, as well as the activity of SOD and POD showed significant differences between the resistant and susceptible strawberry cultivars. The MDA content of the leaves reduced as the resistance strengthened and the activity of CAT increased as the resistance strengthened. These results indicated that the resistance of ‘Jingyu’ to anthracnose was related to the low MDA content, the high content of total soluble proteins and the high activity of SOD, POD and CAT.

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