基于曲线拟合的6种豆的傅里叶变化红外光谱研究

doi:10.11924/j.issn.1000-6850.casb15070043

中国农学通报 ›› 2015, Vol. 31 ›› Issue (32): 81-86.doi: 10.11924/j.issn.1000-6850.casb15070043

基于曲线拟合的6种豆的傅里叶变化红外光谱研究

李会梅,刘刚,马殿旭,欧全宏,于海超,刘艳

(云南师范大学物理与电子信息学院,昆明 650500）

收稿日期:2015-07-07 修回日期:2015-07-28 接受日期:2015-08-11 出版日期:2015-11-16 发布日期:2015-11-16
通讯作者: 刘刚
基金资助:
国家自然科学基金项目“蚕豆生物性病害的振动光谱诊断研究”(30960179)；云南省高校科技创新团队支持计划“云南野生食用菌”。

Study on Six Species of Beans by Fourier Transform Infrared Spectroscopy Based on Curve-fitting

Li Huimei, Liu Gang, Ma Dianxu , Ou Quanhong, Yu Haichao, Liu Yan

(School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500)

Received:2015-07-07 Revised:2015-07-28 Accepted:2015-08-11 Online:2015-11-16 Published:2015-11-16

摘要/Abstract

摘要： 为能够快速的鉴别不同品种的豆，为豆类蛋白、豆类蛋白酶的提取等问题的研究提供研究依据，利用傅里叶变换红外光谱技术结合聚类分析和曲线拟合测试研究6种豆（黄豆、黑豆、蚕豆、红豆、绿豆和红小豆）54个样品的光谱。结果显示，6种豆的红外图谱相似，但在1800~1000 cm^-1范围内红外光谱的峰位、峰形及吸收强度有明显的差异。选取1800~1000 cm^-1范围的光谱图进行二阶导数处理，利用SPSS软件进行聚类分析，聚类分析正确率为100%。利用Origin 8.5软件对1700~1600 cm^-1范围的原始光谱进行曲线拟合处理，6种豆在酰胺Ⅰ带的吸收峰都由9个子峰叠加而成；其中6种豆的蛋白质二级结构中α-螺旋含量、β-转角含量、无规则卷曲结构含量和β-折叠含量不同。结果表明6种豆的蛋白质的二级结构是不同的，傅里叶变换红外光谱(FTIR)结合曲线拟合可以快速简捷地区分不同品种豆类，并能提供豆类所含蛋白质的二级结构的信息。

关键词: 极端最高气温, 极端最高气温, 极端最低气温, 气候变化

Abstract: In order to identify different kinds of beans quickly, provide the basis to extract the beans protein and so on, 54 samples were studied by Fourier Transform Infrared (FTIR) spectroscopy combined with hierarchical cluster analysis (HCA) and curve-fitting analysis. The FTIR results showed that the infrared spectra of the six kinds of beans were similar, but significant differences in wave-numbers and absorption intensities of peaks were observed in the range of 1800-1000 cm^-1. The second derivative spectra in the range of 1800-1000 cm^-1 were selected to perform hierarchical cluster analysis (HCA) and yielded 100% accuracy. The amide I band (1700-1600 cm^-1) in the original spectra was used for decomposition and curve fitting to evaluate the contents of secondary structure of protein. The results showed the amide Ⅰ regions comprised nine sub-bands in the infrared absorbance spectra of beans. The results showed the contents of α-helix, β-turn, unordered structure and β-sheet were dissimilar in the six kinds of beans. It is proved that the secondary structure of protein was different in the six kinds of beans. FTIR spectroscopy combined with curve-fitting analysis could be used to discriminate different kinds of beans rapidly and accurately and provide valuable information about the protein secondary structures of beans.

中图分类号:

O657.3

李会梅,刘刚,马殿旭,欧全宏,于海超,刘艳. 基于曲线拟合的6种豆的傅里叶变化红外光谱研究[J]. 中国农学通报, 2015, 31(32): 81-86.

Li Huimei,Liu Gang,Ma Dianxu,Ou Quanhong,Yu Haichao and Liu Yan. Study on Six Species of Beans by Fourier Transform Infrared Spectroscopy Based on Curve-fitting[J]. Chinese Agricultural Science Bulletin, 2015, 31(32): 81-86.

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基于曲线拟合的6种豆的傅里叶变化红外光谱研究

Study on Six Species of Beans by Fourier Transform Infrared Spectroscopy Based on Curve-fitting

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