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中国农学通报 ›› 2016, Vol. 32 ›› Issue (7): 97-103.doi: 10.11924/j.issn.1000-6850.casb15090092

所属专题: 园艺

• 植物保护 农药 • 上一篇    下一篇

棚栽嫁接西瓜种栽地土壤黄瓜绿斑驳花叶病毒病带毒性研究

蔡美艳,周琳娜,李程巧,冯永斌,叶建人   

  1. 浙江省温岭市植物保护检疫站,浙江省温岭市箬横农林技术推广站,浙江省温岭市植物保护检疫站,浙江省温岭市植物保护检疫站,浙江省温岭市植物保护检疫站
  • 收稿日期:2015-09-22 修回日期:2016-01-21 接受日期:2015-11-25 出版日期:2016-03-18 发布日期:2016-03-18
  • 通讯作者: 蔡美艳
  • 基金资助:
    项目“黄瓜绿斑驳花叶病毒病发生、监测预警和综合防治技术研究”[温科(2013)16 号]。浙江省“三农六方”科技协作计划项目“黄瓜绿斑驳花叶病毒病流行规律与防控技术研究及示范应用”(浙农计发[2012]64 号);温岭市科技

Soil Toxicity of Cucumber Green Mottle Mosaic Virus in Grafted Watermelon Cultured in Greenhouse

  • Received:2015-09-22 Revised:2016-01-21 Accepted:2015-11-25 Online:2016-03-18 Published:2016-03-18

摘要: 为明确棚栽嫁接西瓜地耕作层土壤黄瓜绿斑驳花叶病毒病的带毒情况,以棚栽嫁接西瓜黄瓜绿斑驳花叶病毒病病株根部为中心按向边膜和相邻植株2 个不同方向分别采集植株根部以及距植株根部40、80 cm的表土(0~5 cm)、深土(与表土取样点相对应,距表土23~27 cm深),及取病地不同耕作方式后的土壤,进行双抗体夹心酶联免疫吸附法(DAS-ELLSA)检测以确定土壤的带毒性。结果发现:根围土壤带毒率检测中,健康株与隐症带毒株均不带毒,显症病株中,根部、边膜方向距根部40 cm和80 cm、相邻植株方向距根部40 cm和80 cm,5 个取样点的带毒率表土依次为100%、80%、70%、40%、20%,深土依次为80%、50%、50%、40%、30%;病地不同耕作方式后的土壤带毒检测中,西瓜—水稻—芋头未检测到病毒,西瓜2 年连作与3 年连作带毒率均高于90%,表土自然消解1 年、西瓜—水稻—西瓜、西瓜—蔬菜—西瓜、西瓜—蔬菜—春玉米、西瓜—蔬菜—水稻、西瓜—甘蔗—水稻的带毒率则依次为50%、42.86%、35%、30%、24%、20%。说明病株表土带毒率高于深土,离病株根部越近,或者病株藤蔓覆盖越茂盛,土壤带毒率越高;轮作对病地土壤中的病毒有降解作用。

关键词: 紫薇, 紫薇, 无性系, 生长, 光响应曲线

Abstract: To clarify the soil toxicity of cucumber green mottle mosaic virus disease in the plough layer for greenhouse grafted watermelon, DAS-ELLSA was used for detecting the disease soil. The topsoil (0-5 cm deep) and deep soil (23-27 cm deep) were taken from roots of the infected plants and the adjacent plants, and 40 cm, 80 cm away from the roots to the edge of the membrane. The results showed that in the toxicity test of rhizosphere soil, healthy plants and plants with hidden disease had no virus. In virus infected plants, five sampling points including roots, edge membrane direction 40 cm and 80 cm from the roots, adjacent plants direction 40 cm and 80 cm from the roots, the topsoil virus carried rate was 100%, 80%, 70%, 40%, 20% in turn, deep soil virus carried rate was 80%, 50%, 50%, 40%, 30% in turn. In toxicity test on soil under different tillage methods, watermelon-rice-taro had no virus, continuous cropping of watermelon for two and three years all had high virus carried rate above 90% . The virus carried rate of topsoil biodegrade for one year, watermelon- rice- watermelon, watermelon- vegetable- watermelon, watermelon- vegetable- spring maize, atermelon- vegetable- rice, watermelon-sugarcane-rice was 50%, 42.86%, 35%, 30%, 24% and 20% in turn. So, topsoil virus carried rate was higher than that of deep soil, higher virus carried rate was found in soil which was closer to the root of the infected plant or with more luxuriant infected vine cover, crop rotation had degradation effect on virus infected soil.