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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (16): 161-165.doi: 10.11924/j.issn.1000-6850.casb15110096

所属专题： 园艺

• 食品 营养 检测 安全 • 上一篇    下一篇

石蒜中加兰他敏微波提取工艺研究

周夏禹1,熊海蓉2,张茂美1,王 锦3,熊远福3   

  1. (1常德职业技术学院,湖南常德 415000；2湖南农业大学分析测试中心,长沙 410128；3湖南农业大学理学院,长沙 410128）
  • 收稿日期:2015-11-17 修回日期:2015-12-17 接受日期:2016-01-22 出版日期:2016-06-17 发布日期:2016-06-17
  • 通讯作者: 熊远福＊
  • 基金资助:
    湖南省教育厅科研项目“石蒜药用成分提取与纯化研究”(15C0178)；常德市重点科技项目“魔芋中葡甘聚糖的提取纯化与应用研究”(2014JF15)。

Microwave Extraction Technique of Galanthamine in Lycoris

Zhou Xiayu1, Xiong Hairong2, Zhang Maomei1, Wang Jin3, Xiong Yuanfu3   

  1. (1Changde Vocational Technical College, Changde Hunan 415000;2Center of Analysis and Testing, Hunan Agricultural University, Changsha 410128;3College of Science, Hunan Agricultural University, Changsha 410128)
  • Received:2015-11-17 Revised:2015-12-17 Accepted:2016-01-22 Online:2016-06-17 Published:2016-06-17

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摘要： 石蒜富含重要药用成分加兰他敏，为了优化石蒜中加兰他敏的微波提取工艺，以石蒜为原料、乙醇作提取剂，研究微波功率、料液比、温度、提取时间及次数等单个因素对微波法提取加兰他敏的影响，用正交实验L9(34)确定最佳工艺条件，并与超声波法进行比较。结果显示：用微波法提取石蒜中加兰他敏的最佳条件为料液比1:7，提取温度45℃，微波功率350 W，提取时间7 min；在提取2次的情况下，加兰他敏的提取率达95.8%，产率达0.0549%，粗提取物中加兰他敏含量达15.15%。用微波法提取石蒜中加兰他敏具有提取温度低、时间短、提取率高等优点，整体效果优于超声波法。

关键词: 双季糯高粱, 双季糯高粱, 大豆, 田间配置优化

Abstract: Lycoris has abundant galanthamine which is an important medical constituent. To optimize the microwave extraction technique of galanthamine in Lycoris, influences of single factors including the microwave watt, solid-liquid ratio, temperature, extraction time and times were studied using Lycoris as material and ethanol as extractant. The optimal microwave extraction condition was identified with orthogonal test L9(34), and compared with the ultrasonic method. Results showed that the best extraction condition was as follows: the ratio of sample to solvent was 1:7 (g:mL), the extraction temperature was 45℃, the microwave watt was 350 W, the extraction time was 7 min. After sequential extraction for two times under the condition, the extraction ratio and yield of galanthamine were 95.8% and 0.0549%, respectively, and the concentration of galanthamine in the extract was about 15.15%. Compared with the ultrasonic method, the microwave method had the advantages of lower temperature, less extraction time and higher extraction ratio. The overall effect of microwave extraction was better than that of ultrasonic extraction.