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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (27): 105-108.doi: 10.11924/j.issn.1000-6850.casb16010142

• 生物技术科学 • 上一篇    下一篇

耐镉、铬丝状真菌菌株的筛选与鉴定

丁自立,吴金平,游艾青,汪金平,曹凑贵   

  1. 湖北省农业科学院,湖北省农业科学院,湖北省农业科学院,华中农业大学植物科技学院,华中农业大学植物科技学院
  • 收稿日期:2016-01-29 修回日期:2016-03-07 接受日期:2016-03-21 出版日期:2016-09-29 发布日期:2016-09-29
  • 通讯作者: 曹凑贵
  • 基金资助:
    湖北省科技支撑计划项目“粮田重金属污染情况调查及微生物修复技术研究”(2014BBB001)。

Screening and identification of Resistance to cadmium, chromium filamentous fungi

  • Received:2016-01-29 Revised:2016-03-07 Accepted:2016-03-21 Online:2016-09-29 Published:2016-09-29

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摘要： 采用梯度浓度驯化方法，从重金属镉、铬污染的稻田土壤中分离、筛选出8株耐铬、镉真菌菌株。5株耐镉浓度为16mmol/L的菌株，4株耐铬浓度为1000mg/L的菌株，其中X29菌株耐镉浓度为16mmol/L，耐铬浓度为1000mg/L。经形态观察、18S rDNA扩增和序列分析，确定了这8株菌株的种属地位分别为：绿僵菌(Metarhizium anisopliae)，拟茎点霉(Phomopsis sp.)，曲霉（Aspergillus sp.），木霉（Trichoderma sp.），尖孢镰刀菌（Fusarium oxysporum），酿酒酵母（Saccharomyces cerevisiae），青霉（Penicillium sp.）；其中Strain35和Strain55都为青霉，其余6株分别属于不同菌株。本研究不仅为镉、铬污染环境的微生物修复提供了重要菌株，同时也暗示了微生物在重金属降解和修复中有着良好的应用潜力。

关键词: 西瓜, 西瓜, 枯萎病, 分子辅助选择

Abstract: Eight resistant strains were screened from cadmium and chromium contaminated paddy soil by acclimation of concentration gradient approach. Five strains could endure 16 mmol/L maximum concentration of cadmium (Cd2 ), and four strains could endure 1000 mg/L maximum concentration of chromium (Cr2 ), and X29 strain could endure 16 mmol/L Cd2 and 1000 mg/L Cr2 . The strains were identified from morphological examination and 18S rDNA sequence analysis. Eight filamentous fungi belonged to Metarhizium anisopliae, Phomopsis sp., Aspergillus sp., Trichoderma sp., Fusarium oxysporum, Saccharomyces cerevisiae and Penicillium sp.. Strain35 and Strain55 belonged to Penicillium sp., and the other six strains belonged to different strains. The results not only provided important strains in cadmium and chromium pollution microbial remediation, but also implied that microorganism had good utilization potentiality in heavy metal degradation and remediation.

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