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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (34): 72-79.doi: 10.11924/j.issn.1000-6850.casb16060139

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

火鹤八氢番茄红素脱氢酶基因的克隆与其表达分析

崔金腾1,2,3,田 娜1,张克中1,2,3,4,丰雅茹1   

  1. (1北京农学院园林学院,北京 102206；2城乡生态环境北京实验室,北京 102206；3北京市乡村景观规划设计工程技术研究中心,北京 102206；4北京林果业生态环境功能提升协同创新中心,北京 102206）
  • 收稿日期:2016-06-27 修回日期:2016-08-15 接受日期:2016-08-25 出版日期:2016-12-15 发布日期:2016-12-15
  • 通讯作者: 张克中
  • 基金资助:
    北京市科技提升计划(TJSHG201310020020)；北京市教委科技计划面上项目(KM201510020011)；科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)（市级）(PXM2016_014207_000038)；城乡生态环境北京实验室项目(PXM2016-014207-000003)；北京市属高等学校创新团队建设项目(IDHT20150503)。

Cloning and Expression Analysis of Phytoene Desaturase Gene in Anthurium andraeanum.

Cui Jinteng1,2,3, Tian Na1, Zhang Kezhong1,2,3,4, Feng Yaru1   

  1. (1College of Landscape, Beijing University of Agriculture, Beijing 102206; 2Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 102206; 3Beijing Engineering Research Center of Rural Landscape Planning and Design, Beijing 102206; 4Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit trees, Beijing 102206)
  • Received:2016-06-27 Revised:2016-08-15 Accepted:2016-08-25 Online:2016-12-15 Published:2016-12-15

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摘要： 旨在为火鹤叶色和花色呈色机理研究提供一定理论依据。以火鹤‘阿拉巴马’为材料，提取火鹤肉穗花序中的总RNA，采用RACE技术克隆得到了八氢番茄红素脱氢酶基因(AaPDS)，通过RT-PCR分析其表达变化。八氢番茄红素脱氢酶基因(AaPDS)cDNA全长为2226 bp，其中开放阅读框(ORF)共1767个碱基，编码588个氨基酸。序列比对后发现火鹤AaPDS序列与鹤望兰、水仙、烟草、葡萄、黑藻、向日葵等PDS氨基酸序列存在高度同源性，有74%~79%的一致性。系统进化分析表明火鹤AaPDS与黑藻、水稻、水仙、鹤望兰等单子叶植物的PDS基因聚类在一起应用。RT-PCR分析表明，AaPDS在肉穗花序中表达量最高，在佛焰苞中表达次之，在叶、茎、根表达量依次降低。以上结果可见，通过分子生物学进行系统分类与传统植物表观分类学结果一致。类胡萝素代谢途径中的PDS基因在火鹤花器官成色中起一定作用。

关键词: 小麦, 小麦, 单倍体, 培养基, 激素, 成苗率

Abstract: The aim was to provide a theoretical basis for pigmentation mechanism of leaves and flower of Anthurium andraeanum. Anthurium andraeanum ‘Alabama’ was used as the material, the AaPDS gene was cloned by RACE, and expression analysis was performed by RT-PCR. AaPDS cDNA sequence consisted of 2226 bp with an intact open reading frame of 1767 bp, encoding 588 amino acids. Analysis of sequence alignment indicated that AaPDS sequence had 74%-79% identity with those of Narcissus tazetta, Strelitzia reginae, Nicotiana tabacum, Vitis vinifera, Hydrilla verticillata, Helianthus annuus et al. Phylogenetic analysis indicated that AaPDS was clustered together with those of moncotylous plants, such as Hydrilla verticillata, Oryza sativa, Narcissus tazetta and Strelitzia reginae et al. RT-PCR analysis indicated that AaPDS showed the greatest expression in the spadix, then the expression was gradually reduced in spathe, leaf, stem and root. Taxonomy results obtained by molecular biology were consistent with those by traditional methods. The PDS gene in carotenoids metabolic pathway played some a certain role in the organ pigmentation of Anthurium andraeanum.