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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (26): 1-10.doi: 10.11924/j.issn.1000-6850.casb16080115

所属专题： 马铃薯

• 农学 农业基础科学 •    下一篇

不同耐旱性甘薯不同类型根系的抗氧化特性分析

汪宝卿,姜瑶,解备涛,张海燕,段文学,王庆美,张立明   

  1. 山东省农业科学院作物所,青岛农业大学农学与植物保护学院,山东省农业科学院作物所,山东省农业科学院作物所,山东省农业科学院作物所,山东省农业科学院作物所,山东省农业科学院
  • 收稿日期:2016-08-25 修回日期:2017-09-03 接受日期:2016-09-20 出版日期:2017-09-19 发布日期:2017-09-19
  • 通讯作者: 张立明
  • 基金资助:
    现代农业产业技术体系建设专项资金“国家甘薯产业技术体系北方区栽培岗位”(CARS-11-B-11)；山东省农业科学院青年科研基金“不同 耐旱性甘薯品种苗期不同类型根系的蛋白表达差异分析”(2016YQN04)。

Antioxidant Characteristics of Different Sweetpotato Root Types with Different Drought Tolerance

  • Received:2016-08-25 Revised:2017-09-03 Accepted:2016-09-20 Online:2017-09-19 Published:2017-09-19

摘要： 为揭示不同耐旱性甘薯耐旱生理机制,本试验以耐旱性强的济薯21和耐旱性弱的济紫薯1为材料,在遮雨棚中开展了正常和干旱处理下的甘薯抗氧化特性动态变化研究,结果表明：在干旱条件下下,JS21纤维根、柴根和块根中的H2O2含量和O2—生成速率降幅比JZS1大；无论是正常还是干旱条件下,纤维根中的H2O2含量和O2—生成速率最低,块根中H2O2含量最高,柴根中O2—生成速率最高。在干旱条件下,两个品种的Vc和多酚含量增幅基本持平,但JS21三种类型根中Vc和多酚含量的降幅比JZS1大；无论是正常还是干旱条件下,两个品种中均是柴根中的Vc和多酚含量最高,块根中的Vc和多酚含量最低。无论是正常还是干旱处理下,两个品种中纤维根中SOD、CAT、APX和PPO活性最高,柴根中SOD活性最低,块根中CAT、APX和PPO活性最低；在正常条件下,柴根中的POD活性最高,块根中POD活性最低；在干旱条件下,纤维根的POD活性最高,柴根中的POD活性最低；干旱条件下,从栽插到第三取样期,JS21三种类型根系中SOD、CAT、POD、APX和PPO活性增幅比JZS1大,从第三取样期到第五取样期,JS21三种类型根系中CAT、POD、APX和PPO活性降幅比JZS1小。总之,栽插后70d左右是根系抗氧化能力最强的时间,柴根中抗氧化物质含量高,而纤维根中抗氧化酶活性高,干旱条件下耐旱性越强的品种抗氧化酶增加越快,降低越慢。

关键词: 闽北, 闽北, 中低产田, 障碍因素, 分析

Abstract: In order to revolve physiological mechanism of drought tolerance in sweetpotato, Jishu21 (JS21) with strong drought tolerance and Jizishu1(JZS1) with weak drought tolerance were choosed to research antioxidative character of sweetpotato under normal and drought treatments in rain-shelter. The results showed the decreasing range of H2O2 content and O2— generation rate in fibrous root, thick root, storage root of JS21 was more larger than JZS1. Whether normal or drought treatment, H2O2 content and O2— generation rate were the lowest in fibrous root, H2O2 content was the highest instorage root, and O2— generation rate was the highest in thich root. Under drought condition, the increasing range of Vc and polyphenol content in two varieties were about the same, but the decreasing range of Vc and polyphenol content in three root types of JS21 was more larger than JZS1. Whether normal or drought treatment, Vc and polyphenol content were the highest in thick root, and the lowest in storage root. The activities of SOD, CAT, APX and PPO were the highest in fibrous root of two varieties, but CAT, APX and PPO were the lowest in storage, and SOD were the lowest in thick root. Under normal treatment, the activity of POD was the highest in thick root, but lowest in storage root. Under drought treatment, the activity of POD was the highest in fibrous root, but lowest in thick root, and the increasing range of SOD, CAT, POD, APX and PPO in three root types of JS21 were more lager than JZS1 from transplanting to 3rd sampling date, the decreasing range of CAT, POD, APX and PPO in three root types of JS21 were more smaller than JZS1 from 3rd sampling date to 5th sampling date. In conclusion, 70 days after transplanting maybe was the time of the highest antioxidative capacity, antioxidant content was higher in thick root, but antioxidantive enzymes were higher in fibrous root, antioxidative enzymes of the variety with stronger drought tolerance increased more faster and decreased more slower under drought condition.