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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (15): 144-149.doi: 10.11924/j.issn.1000-6850.casb17020094

• 食品 营养 检测 安全 • 上一篇    下一篇

DNS法定量测定还原糖的波长选择

廖祥兵1,2,陈晓明1,2,肖 伟1,3,张祥辉1,田 甲1   

  1. (1西南科技大学生命科学与工程学院,四川绵阳 621010；2国民核生化灾害防护国家重点实验室,北京 102205；3核废物与环境安全国防重点学科实验室,四川绵阳 621010）
  • 收稿日期:2017-02-23 修回日期:2017-05-05 接受日期:2017-03-22 出版日期:2017-05-26 发布日期:2017-05-26
  • 通讯作者: 肖伟
  • 基金资助:
    国民核生化灾害防护国家重点实验室开发基金项目“铀污染的牧草-微生物联合修复”(SKLNBC 2015-04)；核废物与环境安全国防重点实验室基金“铀污染的生物预警及修复”(15yyhk05)；校博士基金项目“可视化重金属（铀）污染微生物预警体系的构建与应用” (16ZX7113)。

The Wavelength Selection of Reducing Sugar Quantitatively Determined by DNS Method

Liao Xiangbing1,2, Chen Xiaoming1,2, Xiao Wei1,3, Zhang Xianghui1, Tian Jia1   

  1. (1College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang Sichuan 621010;2State key Laboratory of NBC Protection, Beijing 1022053The Key Laboratory of Fundamental Science on Nuclear Wasters and Environment Safety, Mianyang Sichuan 621010)
  • Received:2017-02-23 Revised:2017-05-05 Accepted:2017-03-22 Online:2017-05-26 Published:2017-05-26

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摘要： 为研究3,5-二硝基水杨酸(DNS)比色法定量检测还原糖的最佳测定波长。采用紫外可见分光光度计对样品进行扫描，将每个波长下不同浓度显色液的吸光值与还原糖浓度进行线性拟合并分析，另通过对同一样品的10次扫描结果的RSD进行分析以验证波长选择是否合理。结果发现：葡萄糖在520 nm处线性相关系数 ≈ ，在520~580 nm范围内R2稳定在0.9998且在范围内斜率随波长增大而减小。半乳糖在520 nm处线性相关系数也存在 ≈ ，而在520~580 nm范围内R2及斜率随波长增大而减小。对同一样品的相对标准偏差(RSD)进行分析，发现RSD520≈RSDmin。此外，在480~580 nm波长范围内对照组吸光值都较为稳定，而试验组吸光值在该范围短波长区域波动明显，且波长越小波动越大。结果表明在520 nm处既可达到很好的线性关系，又可实现较好的分辨率，同一样品的验证实验很好的验证了该结论。试验组吸光值在较短波长处的波动表明选择较短波长是不合理的，同时说明新生成的氨基化合物的光吸收基团和DNS原有的光吸收基团为不同基团。

关键词: 青海湖南部, 青海湖南部, 云量, 气候要素, 影响, 研究

Abstract: The aim is to get the optimum determination wavelength of reducing sugar quantitatively determined by DNS method. The samples were scanned by UV visible spectrophotometer, and then the absorbance and the concentration of reducing sugar were linearly fitted and analyzed at each wavelength. And to verify whether the wavelength selection is reasonable, the RSD of 10-time scan results of the same sample was analyzed. The results showed that the linear correlation coefficient (R2) of glucose was very close to R2 max at 520 nm ( R2 520 ≈ R2 max ). In the range of 520-580 nm, R2 was stable at 0.9998 and the slope decreases with the increase of wavelength. R2 of galactose was also very close to the R2 max at 520 nm ( R2 520 ≈ R2 max ). In the range of 520- 580 nm, R2 and the slope also decreased with the increase of wavelength. The results of RSD analysis of the same sample showed RSD520≈RSDmin. In addition, the absorbance value of control group was stable in the range of 480-580 nm, while the absorbance value of the experimental group fluctuated significantly in the short wavelength range, the smaller the wavelength, the greater the fluctuation. The results showed that the better linear relationship and resolution could be got at 520 nm, which was verified by the verified experiment of the same sample. The absorbance fluctuation of the experimental group showed that it was unreasonable to choose a shorter wavelength; it also showed that the light absorbing group of the newly formed amino compound was different from the original light absorbing group of the DNS.

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