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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (9): 57-61.doi: 10.11924/j.issn.1000-6850.casb17040037

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

无核葡萄种质亲缘关系的SRAP研究

史圆圆,张旭彤,王跃进,张朝红   

  1. 西北农林科技大学园艺学院/农业部西北地区园艺作物生物学与种质创制重点实验室,西北农林科技大学园艺学院/农业部西北地区园艺作物生物学与种质创制重点实验室,西北农林科技大学园艺学院/农业部西北地区园艺作物生物学与种质创制重点实验室,西北农林科技大学园艺学院/农业部西北地区园艺作物生物学与种质创制重点实验室
  • 收稿日期:2017-04-08 修回日期:2017-05-13 接受日期:2017-05-25 出版日期:2018-03-21 发布日期:2018-03-21
  • 通讯作者: 张朝红
  • 基金资助:
    国家现代农业产业技术体系建设专项“无核葡萄育种”(CARS-30-yz-7)；杨凌示范区农业科技示范推广项目“葡萄优质安全生产关键技术 集成与示范推广”(2015-TS-10)。

Genetic Relationship of Seedless Grape Germplasm by SRAP Markers

  • Received:2017-04-08 Revised:2017-05-13 Accepted:2017-05-25 Online:2018-03-21 Published:2018-03-21

摘要： 为充分了解及更合理地利用无核葡萄的种质资源，辅助无核葡萄育种，本研究采用SRAP分子标记技术，对23 个国内外主流无核葡萄品种的种质亲缘关系进行分析。结果表明：从45 对SRAP引物中筛选出38 对多态性引物，共扩增出236 条谱带，其中171 条为多态性谱带，多态性谱带百分率为72.4%。遗传相似分析显示，供试材料间遗传相似系数的变化范围为0.58~0.90，遗传距离在1.0313~4.9643 范围内变化。在遗传相似系数为0.61 处，可将供试材料分为2 个大类群，在相似系数为0.66 处，Ⅰ类群和Ⅱ类群均可分为2个亚群。说明23个品种总体来讲亲缘关系较远，特别欧美杂种具有较高的遗传多样性，部分欧亚种品种间亲缘关系较近，杂交育种时应注意亲本选择。

关键词: 傅里叶变换红外光谱, 傅里叶变换红外光谱, 豆, 二阶导数光谱, 聚类分析, 曲线拟合

Abstract: To know and utilize seedless grape germplasm resources, and assist seedless grape breeding, the genetic relationships among 23 seedless grape cultivars from home and abroad were studied by sequence related amplified polymorphism (SRAP) markers in this study. The results showed that 38 polymorphic primer combinations were selected from 45 primer pairs, which amplified 236 bands, 171 bands of which were polymorphic and the percentage of polymorphic bands was 72.4%. Analysis of genetic similarity showed that the genetic similarity coefficients ranged from 0.58 to 0.90, and the variation range of genetic distances was between 1.0313 and 4.9643. When the genetic similarity coefficient was 0.61, these seedless grape cultivars could be clustered into two groups. Furthermore, when the threshold was 0.66, both group I and group Ⅱ could be classified into two subgroups. It indicated that the genetic relationships among the 23 cultivars were far, there was abundant genetic diversity among Euro-American hybrids grape, while part ofvitis vinifera had close relationships. So the selection of parents should be paid attention to in cross breeding.