高渗透压胁迫下水稻干尖线虫Ab-tre基因表达

doi:10.11924/j.issn.1000-6850.casb17060063

中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 141-146.doi: 10.11924/j.issn.1000-6850.casb17060063

所属专题：生物技术；水稻

高渗透压胁迫下水稻干尖线虫Ab-tre基因表达

陈俏丽,王峰,李丹蕾,零雅茗,张瑞芝

东北林业大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学

收稿日期:2017-06-09 修回日期:2018-08-02 接受日期:2017-09-21 出版日期:2018-08-28 发布日期:2018-08-28
通讯作者: 王峰
基金资助:
国家自然科学基金“Bx-HSF1 在松材线虫DL3 形成过程中的转录调控机理研究”(31570637)。

Gene Expression of Aphelenchoides besseyi trehalase (Ab-tre) Gene under Hypertonic Osmotic Stress

Received:2017-06-09 Revised:2018-08-02 Accepted:2017-09-21 Online:2018-08-28 Published:2018-08-28

摘要/Abstract

摘要： 水稻干尖线虫可通过海藻糖酶水解海藻糖提供能量以适应高渗透压胁迫，本研究旨在探究水稻干尖线虫海藻糖酶基因（Ab-tre）在高渗透压胁迫下的表达。在转录组测序结果基础上，克隆到水稻干尖线虫海藻糖酶基因，以30%甘油溶液为高渗透压胁迫剂，通过荧光定量Q-PCR及原位杂交探究该基因在高渗透压胁迫下的表达。荧光定量Q-PCR表明，高渗透压胁迫3~7 min，及高渗透压胁迫140 min后，在蒸馏水中恢复90 min时，Ab-tre表达量均有显著上调。原位杂交表明，Ab-tre主要在水稻干尖线虫体中部有杂交信号。本研究表明Ab-tre与水稻干尖线虫抗高渗透压胁迫过程相关，对Ab-tre功能进行研究，可为防治该线虫奠定理论基础。

关键词: 苦荞种质资源, 苦荞种质资源, 生物性性状, 主成分分析, 聚类分析

Abstract: Trehalose can be hydrolyzed by trehalase in rice white tip nematode (Aphelenchoides besseyi ), which provides energy to enable the survival under hypertonic osmotic stress. This study aims at studying the gene expression of A. besseyi trehalase (Ab- tre ) gene. Ab- tre was cloned based on the transcriptome sequencing result. A 30% glycerol solution was selected to be the dehydrant to provide a hypertonic osmotic stress and the expression ofAb-tre under the hypertonic osmotic stress was studied by Q-PCR and in situ hybridization. Q- PCR showed that the gene expression of Ab- tre increased significantly when the nematodes were undergoing hypertonic osmotic stress for 3-7 min or recovered in distilled water for 90 min after a 140 min treatment of hypertonic osmotic stress. In situ hybridization showed thatAb-tre was mainly located in the central of the nematode. This study indicated that Ab-tre was related to hypertonic osmotic stress resistance ofA. besseyi . The study on the function ofAb-tre can provide a theoretical basis for the prevention and control of the nematode.

中图分类号:

S432.1

陈俏丽,王峰,李丹蕾,零雅茗,张瑞芝. 高渗透压胁迫下水稻干尖线虫Ab-tre基因表达[J]. 中国农学通报, 2018, 34(24): 141-146.

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高渗透压胁迫下水稻干尖线虫Ab-tre基因表达

Gene Expression of Aphelenchoides besseyi trehalase (Ab-tre) Gene under Hypertonic Osmotic Stress

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