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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (20): 151-157.doi: 10.11924/j.issn.1000-6850.casb17120093

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

不动杆菌BCL-1固体发酵条件优化及其对茶鲜叶吡虫啉的降解

胡桂萍,石旭平,黎小萍,杨广,曹红妹,施龙清,张建华,欧阳雪灵,杨帆   

  1. 江西省蚕桑茶叶研究所,江西省蚕桑茶叶研究所,江西省蚕桑茶叶研究所,福建农林大学,江西省蚕桑茶叶研究所,福建农林大学应用生态所,江西省蚕桑茶叶研究所,江西省蚕桑茶叶研究所,江西省蚕桑茶叶研究所
  • 收稿日期:2017-12-22 修回日期:2018-06-19 接受日期:2018-03-23 出版日期:2018-07-13 发布日期:2018-07-13
  • 通讯作者: 胡桂萍
  • 基金资助:
    国家重点研究计划“农业土壤治理与改良关键技术的联合研发”(2016YFE0102100)；江西省重点研发计划项目“基于农药残留控制的茶叶 安全生产关键技术联合研发与示范”(20161BBH80071)；闽台作物有害生物生态防控国家重点实验室开放课题基金(SKB2017011)。

Acinetobacter sp. BCL-1: Optimization of Its Solid Fermentation and Imidaclorpid Remediation Potential on Tea Leaves

黎小萍,,,,, and 杨帆   

  • Received:2017-12-22 Revised:2018-06-19 Accepted:2018-03-23 Online:2018-07-13 Published:2018-07-13

摘要： 为了优化吡虫啉降解菌固态发酵条件及确定适宜固态发酵基质,以菌株生物量为指标,筛选固体发酵培养基配方,通过单因素分析接种量、发酵温度、发酵时间对菌株生物量的影响,并利用响应面分析法优化最佳发酵条件,同时进行菌制剂茶叶鲜叶吡虫啉降解小区试验分析。结果表明：不动杆菌BCL-1固体发酵适宜培养基配方为：草木灰65%+玉米粉8%+麸皮24%+葡萄糖2.3%+磷酸二氢钾0.5%+硫酸镁0.2%；适宜发酵条件为：发酵时间72.80h,接种量5.04%,发酵温度28.89℃。在此条件下,生物量可以达到9.537×108 cfu/g。菌制剂可以有效降解茶叶鲜叶上的吡虫啉,施用12天后,茶叶鲜叶降解率达到82.0%。

关键词: 小麦, 小麦, 低温冻害, 土壤含水量, 植株性状, 产量

Abstract: The solid-fermentation conditions of imidacloprid-degrading strain and its suitable solid-fermentation medium composition were optimized and determined in this paper, with the index of the biomass. Effects of the fermentation conditions, such as medium component, inoculation, fermentation time and temperature, were discussed by a single factor experiment, and the optimal fermentation condition of solid fermentation was determined by using response surface analysis, and then the remediation effects of microbial agents on imidaclorprid on the tea leaf were also studied. The results showed that the optimal fermentation conditions were as follows: plant ash 65%, corn pink 8%, bran 24%, glucose 2.3%, KH2PO3 0.5%, MgSO4 0.2%; fermentation time 72.8h, inoculum amount 5.04%, fermentation temperature 28.89℃. Under these conditions, the number of biomass researched 9.537×108 cfu/g. After 12 days, degradation rate of imidacloprid on the tea leaf reached 82.0%, showing the extent imidacloprid remediation potential.