灭多威胁迫下罗非鱼精巢转录组变化及信号通路分析

doi:10.11924/j.issn.1000-6850.casb18040125

中国农学通报 ›› 2019, Vol. 35 ›› Issue (28): 44-51.doi: 10.11924/j.issn.1000-6850.casb18040125

所属专题：生物技术；水产渔业

灭多威胁迫下罗非鱼精巢转录组变化及信号通路分析

孟顺龙

中国水产科学研究院淡水渔业研究中心

收稿日期:2018-04-25 修回日期:2018-07-31 接受日期:2018-08-24 出版日期:2019-10-14 发布日期:2019-10-14
通讯作者: 孟顺龙
基金资助:
：中国水产科学研究院淡水渔业研究中心基本科研业务费“养殖水体中灭多威的消解动态及微生物去除技术研究”(2017JBFM07)；江苏省青年自然科学基金资助“环境雌激素类农药灭多威胁迫下罗非鱼精巢转录组变化及基因通路分”(BK20150117)；现代农业产业技术体系建设专项资金“特色淡水鱼产业技术体系”(CARS-46)。

Testis of Male Tilapia Under Methomyl Stress: Transcriptome Changes and Signal Pathway Analysis

Received:2018-04-25 Revised:2018-07-31 Accepted:2018-08-24 Online:2019-10-14 Published:2019-10-14

摘要/Abstract

摘要： 应用高通量测序技术(RNA-seq),获得显著差异表达基因369条,160条基因显著下调,209条基因显著上调。其中显著差异表达基因在ECM与受体相互作用信号通路和MAPK信号通路中富集明显。分析发现,灭多威对精巢组织细胞粘附性造成影响,诱导ECM与受体相互作用中相关基因表达显著上调,如层粘连蛋白Laminins、整合素ɑ6、β1等,以稳定精巢组织结构；JNK途径中MAP3K、MKK4、JNK显著表达上调,增强录因子AP-1,促进 P53、Bax等促凋亡蛋白的表达；MAPK信号通路ERK途径中相关生长因子表达受到影响,从而可能对罗非鱼精巢组织生殖细胞增殖产生影响。

关键词: 黄秋葵, 黄秋葵, 施肥, 生长, 果荚

Abstract: 369 significantly expressed genes were obtained by RNA sequencing(RNA-Seq). 160 genes were significantly down- regulated, and 209 genes were up-regulated. The expression of the differentially expressed genes in MAPK signaling pathway and ECM-receptor interaction was more significant. The results indicated that the adhesion of Testis tissue cells was influenced by Methomy stress, that induced the expression of related gene in ECM-receptor interaction to up-regulated for stabilizing the structure of testis tissue, such as Laminins, integrin alpha 6 and integrin beta 1. JNK, MAP3K and MKK4 in JNK pathway were significantly up-regulated, this enhanced transcription factor AP-1 to promote the expression of P53, Bax and other pro-apoptotic proteins. Because the expression of related growth factors in the ERK pathway of MAPK signaling pathway was significantly down-regulated by methomy stress, the proliferation of reproduction cells in the testis tissue may be affected.

孟顺龙. 灭多威胁迫下罗非鱼精巢转录组变化及信号通路分析[J]. 中国农学通报, 2019, 35(28): 44-51.

参考文献

Anders S, Pyl P T, Huber W. HTSeq-A Python framework to work with high-throughput Sequencing data[J]. Bioinformatics, 2015, 31(2): 166.
Basciani S, Mariani S, Spera G, et al. Role of platelet-derived growth factors in the testis[J]. Endocr Rev, 2010, 31: 916–939.
Biolly B, Vercouter-Edouart AS, Hondermark H, et al. FGF singnals for cell proliferation and migration through different pathways[J]. Cy-tokine Growth Factor Rev, 2000, 1(4): 295-302.
El-Saeid M H, Al-Turki A M, Al-Wable M I, et al. Evaluation of pesticide residues in Saudi Arabia ground water [J].Research Journal of Environmental Sciences, 2011, 5(2): 171-178.
Eilers A, Whitfield J, Shah B, et a1. Direct inhibition of cJun N— terminal kinase in sympathetic neurones prevents c-jnil promoter activation and NGF withdrawal-induced death[J]. J Neurochem.200l, 76 (5): 1439-1454.
Goodman SL, Picard M. Integrins as therapeutic targets[J]. Trends Pharmacol Sci, 2012, 33(7): 405-412.
Lee NP, Cheng CY. Ectoplasmic specialization, a testis-specific cell-cell actin-based adherens junction type: is this a potential target for male contraceptive development?[J]. Hum Reprod Update, 2004, 10(4):349-369.
Marcel M, Michael E, Wiliam E A, et al. Genome Sequencing in Open Microfabricated High Density Picoliter Reactors[J]. Nature, 2005, 437(7057):376-380.
Meng S L, Qiu L P, Hu G D, et al. Effects of methomyl on steroidogenic gene transcription of the hypothalamic-pituitary-gonad-liver axis in male tilapia [J]. Chemosphere, 2016a, 165:152-162.
Meng S L, Qiu L P, Hu G D, et al. Responses and recovery pattern of sex steroid hormones in testis of Nile tilapia (Oreochromis niloticus) exposed to sublethal concentration of methomyl [J]. Ecotoxicology, 2016b, 25(10):1-7.
Meng S L, Qiu L P, Hu G D, et al. Effect of methomyl on sex steroid hormone and vitellogenin levels in serum of male tilapia (Oreochromis niloticus) and recovery pattern [J]. Environmental Toxicology, 2017, 32:1869-1877.
Robinson MD, Mccarthy DJ, SmythGK . edge R: a Bioconductor package for differential expression analysis of digital gene expression data[J]. Bioinformatics. 2010, 26(1): 139-40.
Sun L, Lee H K. Stability studies of propoxurher-bisice in environmental water samples by liquid chromatography [J]. Journal of Chromatography, 2003, 10(14): 153- 163.
Tim R Mercer, Irfan A Qureshi, Solen Gokhan, etal. Long noncoding RNAs in neuronal-glial fate specification and oligodendrocyte lineage maturation [J]. BMC Neuroscience. 2010, 11(1):14.
Trapnell C, Pachter L, Salzberg S L. Top Hat: discovering splice junctions with RNA-Seq[J]. Bioinformatics, 2009, 25(9): 1105–1111.
U.S.EPA. 2012 Edition of the Drinking Water Standards and Health Advisories. EPA 822-S-12-001. Office of WaterU.S. Environmental Protection Agency, Washington, DC. 2012.
Van-Scoy A R, Yue M, Deng X, et al. Environmental fate and toxicology of methomyl [J]. Reviews of environmental contamination and toxicology, 2013, 222: 93-109.
Wang S F, Yen J C, Yin P H, el at. Involvement ofoxidative stress-activated JNK signaling in the methamphetamine-induced cell death of human SH—SY5Y cells[J]. Toxicology, 2008, 246 (2-3): 234-241.
Weston C R, Davis R J. The JNK signal transduction pathway[J]. Curr Opin Genet Dev, 2002, 12(1): 14-21.
傅应亚, 黎友伦. JNK信号通路与细胞凋亡[J]. 重庆医科大学学报, 2014, 39(3): 281-283.
郭新彪.环境健康学[M].北京: 北京大学医学出版社, 2006.
虎明明, 刘秀芳, 关霞, 等. 辛硫磷和灭多威对雌性大鼠生殖系统的联合毒性作用[J]. 癌变.畸变.突变, 2008, 20(6): 470-474.
何瑞玲, 齐景凯, 张玉芬, 等. 通辽地区大米农药残留量调查[J]. 中国公共卫生管理, 2007, 23(5): 464-466.
孟顺龙. 环境雌激素灭多威对雄罗非鱼下丘脑-垂体-性腺轴和抗氧化防御系统的影响研究[D]. 南京农业大学, 2014.
王静, 刘铮铮, 潘荷芳, 等. 浙江省市级饮用水源地氨基甲酸酯农药的分析法、污染特征及健康风险研究[J]. 环境化学, 2010, 29(4): 623-628.
张学健, 程永红, 李世荣. 固相萃取/高效液相色谱法测定生活饮用水中氨基甲酸酯类农药[J]. 中国卫生检验杂志, 2014, 24(9): 1246-1247.
张冰, 傅晓钦, 徐能斌, 等. 液相色谱-质谱法测定水中氨基甲酸酯类农药及其代谢物[J]. 化工环保, 2010, 30(4): 364-367.
张春兰, 王桂芝, 秦孜娟, 等. 转录组与RNA-Seq技术[J]. 生物技术通报, 2012(12): 51-56.

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灭多威胁迫下罗非鱼精巢转录组变化及信号通路分析

Testis of Male Tilapia Under Methomyl Stress: Transcriptome Changes and Signal Pathway Analysis

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