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中国农学通报 ›› 2020, Vol. 36 ›› Issue (1): 131-134.doi: 10.11924/j.issn.1000-6850.casb20190600237

所属专题: 烟草种植与生产

• 植物保护·农药 • 上一篇    下一篇

黄花蒿烟草醋液对三线镰刀菌生长的抑制作用

张晓云, 李维蛟()   

  1. 云南中医药大学,昆明 650500
  • 收稿日期:2019-06-05 修回日期:2019-06-20 出版日期:2020-01-05 发布日期:2020-01-07
  • 通讯作者: 李维蛟
  • 作者简介:张晓云,女,1979年出生,山西浑源人,助理研究员,博士,研究方向为药用资源开发利用。通信地址:650500 云南省昆明市呈贡新区雨花路1076号 云南中医药大学,E-mail:hellozhangxy@126.com。
  • 基金资助:
    云南省科学技术厅“云南省道地濒危中药材繁育与栽培工程技术研究中心”(2016DH011);云南省科学技术厅“云南省重大科技专项生物医药砂仁规范化种植关键技术研究与应用”(2018ZF011-0202)

Effect of Mixed Artemisia annua and Nicotiana tabacum Vinegar on Fusarium tricinctum

Zhang Xiaoyun, Li Weijiao()   

  1. Yunnan University of Chinese Medicine, Kunming 650500
  • Received:2019-06-05 Revised:2019-06-20 Online:2020-01-05 Published:2020-01-07
  • Contact: Li Weijiao

摘要:

充分利用黄花蒿烟草秸秆制成秸秆混合醋液,并初步研究其对三线镰刀菌生长的影响。分离纯化真菌并对菌种进行传统形态学和分子学鉴定,利用干馏法制取黄花蒿烟草秸秆醋液,设置浓度梯度观察黄花蒿烟草秸秆醋液对三线镰刀菌2株菌的生长影响。鉴定获得的其中2株菌种均为三线镰刀菌,且在黄花蒿烟草秸秆醋液50%浓度下,生长抑制率的相对校正率近似为100%,达到完全被抑制的效果。研究结果为黄花蒿烟草秸秆潜在的利用价值研究及开发提供技术参考。

关键词: 黄花蒿烟草秸秆, 醋液, 鉴定, 抑菌

Abstract:

The mixed vinegar was made from straw of Artemisia annua and Nicotiana tabacum, and its effect on the growth of Fusarium tricinctum was studied. Fungi were isolated and purified, and strains were identified using the methods of traditional morphological and molecular. The straw vinegar of A. annua and N .tabacum was prepared by distillation method. Concentration gradient was set to observe the effects of the straw vinegar on the growth of these two fungi. The two strains were both identified as F. tricinctum. The relative correction rate of the growth inhibition rate was approximately 100% at 50% concentration of the vinegar, which achieved the effect of complete inhibition. The study provides technical references for the research and development of the potential utilization value of straw of A. annua and N. tabacum.

Key words: straw of Artemisia annua and Nicotiana tabacum, vinegar, identification, antimicrobial activities

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