关键词: 生态可持续, 生态可持续, 土地利用, 格局分配

Abstract: To explore the feasibility of InDel molecular marker technology for identifying the uniformity of sugar beet varieties, 8 sugar beet varieties were selected as test materials, and 24 individuals per variety were respectively sampled to extract genomic DNA. Subsequently, 9 pairs of InDel primers located in 9 chromosomes were employed for InDel-PCR amplification and the uniformity of each variety was assessed based on amplified bands. InDel-PCR amplification results showed that the average uniformity rate in 8 sugar beet varieties was from 67.7% to 88.1%. The same primer presented different uniformity rates in 8 sugar beet varieties. For example, the uniformity rate detected by ND286 primer in variety 985 and variety 1123 reached 100%, but the uniformity rate detected in other varieties distributed from 46% to 63%. Different primers might show the same uniformity rate in the same variety, but the uneven plants may be the same or not. For example, the uniformity rate detected by ND31 and ND71 primers was the same in variety 1015, which gained the highest average uniformity rate. However, plantlet 13, 14 and 15 were identified as uneven plants by ND31 primers, while plantlet 8, 15 and 21 were identified by ND71 primers. Therefore, uniformity identification of sugar beet varieties by single InDel primer is not universal and scientific. It is ecessary to integrate the amplification results at multiple InDel loci for identifying the uniformity of sugar beet varieties, and the criteria for identifying the uniformity of sugar beet varieties should not be set too strict at this stage.