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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (3): 61-65.doi: 10.11924/j.issn.1000-6850.casb2020-0446

所属专题： 园艺

• 农学·农业基础科学 • 上一篇    下一篇

利用分子标记快速鉴定甜菜育性的研究

石好琪(), 丁刘慧子, 邳植, 吴则东()   

  1. 黑龙江大学现代农业与生态环境学院,哈尔滨 150080
  • 收稿日期:2020-09-10 修回日期:2020-11-16 出版日期:2021-01-25 发布日期:2021-01-26
  • 通讯作者: 吴则东
  • 作者简介:石好琪,男,1997年出生,河南驻马店人,硕士研究生,研究方向：甜菜遗传及分子育种。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学现代农业与生态环境学院,Tel：18238771911,E-mail： 3091963535@qq.com。
  • 基金资助:
    现代农业产业技术体系建设专项资金(cars-170111)

Sugar Beet Fertility: Rapid Identification by Using Molecular Markers

Shi Haoqi(), Ding Liuhuizi, Pi Zhi, Wu Zedong()   

  1. College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2020-09-10 Revised:2020-11-16 Online:2021-01-25 Published:2021-01-26
  • Contact: Wu Zedong

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摘要：

试验旨在优化分子标记鉴定甜菜育性的整个鉴定过程,为分子标记鉴定甜菜育性真正应用于实际育种工作打下基础。以随机抽取的10份糖甜菜和5份甜菜多胚种质资源嫩叶为试材,采用两种甜菜DNA提取方法、双重PCR以及两种电泳方式对甜菜育性进行鉴定。结果表明,裂解液法在提取甜菜DNA上更加高效便捷,1 h就可以提取100份甜菜DNA,效率远远高于CTAB法;采用双重PCR的方式进行甜菜育性鉴定完全可行,使甜菜育性鉴定时间减半。对两种电泳方式进行对比发现,6%聚丙烯酰胺凝胶电泳跑出的结果不能满足实验要求,而1%琼脂糖凝胶电泳跑出的结果完全满足实验要求。通过对用分子标记鉴定甜菜育性的整个鉴定体系进行优化,得出用裂解液法进行甜菜DNA的提取、双重PCR进行甜菜育性的鉴定、电泳方式选用1%琼脂糖凝胶电泳可以达到快速鉴定甜菜育性的目的。

关键词: 甜菜, 分子标记, 育性鉴定, 快速鉴定, DNA提取

Abstract:

The aim of the experiment is to optimize the entire identification process of sugar beet fertility with molecular markers and lay a foundation for the real application of molecular markers in identifying sugar beet fertility in actual breeding work. We identified the fertility of sugar beet with two methods of DNA extraction from sugar beet, double PCR and two electrophoresis, with 10 randomly selected young leaves of sugar beet and 5 leaves of multigerm germplasm resources of sugar beet as test materials. The results showed that the lysis solution method was more efficient and convenient in extracting DNA from sugar beet and 100 parts of DNA from sugar beet were extracted in 1 h, which was much more efficient than the CTAB method; the double PCR method was completely feasible for fertility identification of sugar beet, the time required for identifying sugar beet fertility was reduced by half. Comparing the two electrophoresis methods, it was found that the 6% polyacrylamide gel electrophoresis did not meet the experimental requirements, while the 1% agarose gel electrophoresis fully met the experimental requirements. By optimizing the whole identification system for identifying the fertility of sugar beet with molecular markers, it is concluded that the DNA extraction from sugar beet with lysis solution method, the identification of the fertility of sugar beet by double PCR and 1% agarose gel electrophoresis could achieve the purpose of rapid fertility identification of sugar beet.

Key words: sugar beet, molecular marker, fertility identification, rapid identification, DNA extraction

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