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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (27): 122-128.doi: 10.11924/j.issn.1000-6850.casb20200300268

• 生物科学 • 上一篇    下一篇

3株氟磺胺草醚降解真菌的分离与鉴定

潘国强1,2,3(), 杨峰山1,2,3, 王家瑞1,2,3, 汤元豪1,2,3, 马玉堃1,2,3, 付海燕1,2,3, 刘春光1,2,3()   

  1. 1黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500
    2黑龙江大学生命科学学院,黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨 150080
    3黑龙江大学生命科学学院,黑龙江省普通高校微生物重点实验室,哈尔滨 150080
  • 收稿日期:2020-03-27 修回日期:2020-05-17 出版日期:2020-09-25 发布日期:2020-09-23
  • 通讯作者: 刘春光
  • 作者简介:潘国强,男,1999年出生,黑龙江哈尔滨人,本科,研究方向:长残效除草剂的微生物降解与土壤修复。通信地址:150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学生命科学学院,Tel:0451-86608586,E-mail: 1435297256@qq.com。
  • 基金资助:
    黑龙江省自然科学基金“寒地黑土微生物响应阿特拉津残留群落结构与多样性变化研究”(面上项目:C2018051);黑龙江省普通本科高等学校青年创新人才培养计划“阿特拉津降解复合菌肥研发与农田土壤微生物修复”(UNPYSCT-2017119)

Three Strains of Fomesafen Degrading Fungi: Isolation and Identification

Pan Guoqiang1,2,3(), Yang Fengshan1,2,3, Wang Jiarui1,2,3, Tang Yuanhao1,2,3, Ma Yukun1,2,3, Fu Haiyan1,2,3, Liu Chunguang1,2,3()   

  1. 1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
    2Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080
    3Key Laboratory of Microbiology, College of Heilongjiang, Heilongjiang University, Harbin 150080
  • Received:2020-03-27 Revised:2020-05-17 Online:2020-09-25 Published:2020-09-23
  • Contact: Liu Chunguang

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摘要：

为了解决大豆田除草剂氟磺胺草醚长期使用造成的残留问题,本研究利用富集培养法进行菌株筛选,利用高效液相色谱法测定其降解能力,通过形态和16S rDNA序列分析对其进行种属鉴定。结果表明,本研究从长期施用氟磺胺草醚的土壤中分离纯化出3株能以氟磺胺草醚为唯一碳源生长的真菌菌株FF1、FF2和FF3。它们在7天内对初始浓度600 mg/L氟磺胺草醚的降解率分别为21.03%、15.74%和11.88%,这3个菌株分别鉴定为Aspergillus jensenii（詹森曲霉）、Penicillium dipodomyicola（双足青霉）和Rhizopus oryzae（稻根霉菌）。这3个种属的获得为氟磺胺草醚污染土壤生物修复提供了新的菌种资源。

关键词: 氟磺胺草醚, 真菌, 分离, 鉴定, 降解

Abstract:

In order to solve the problem of fomesafen residues in soybean field caused by the long-term application of the herbicide fomesafen, the enrichment and acclimation method was used to screen the strains, and the high efficiency liquid chromatography method was used to determine the degradation ability, and the species were identified by morphological and 16S rDNA sequence analysis in this study. The results showed that three fungal strains FF1, FF2 and FF3, which grew with fomesafen as the only carbon source, were isolated and purified from the soil with long-term application of fomesafen. Their degradation efficiency of the initial concentration 600 mg/L fomesafen in seven days was 21.03%, 15.74% and 11.88%, respectively. These three strains were identified as Aspergillus jensenii, Penicillium dipodomyicola and Rhizopus oryzae. The acquisition of the three species could provide new resources for bioremediation of contaminated soil with fomesafen.

Key words: fomesafen, fungus, isolation, identification, degradation

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