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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (4): 98-102.doi: 10.11924/j.issn.1000-6850.casb2023-0139

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

基于SSR的云南野生猕猴桃种质资源亲缘关系分析

王连润(), 万红, 陶磅, 陈霞, 李坤明, 沙毓沧, 丁仁展()   

  1. 云南省农业科学院园艺作物研究所，昆明 650205
  • 收稿日期:2023-02-21 修回日期:2023-10-31 出版日期:2024-02-05 发布日期:2024-01-29
  • 通讯作者:
    丁仁展，男，1972年出生，山东牟平人，研究员，硕士，主要从事果树资源及栽培工作。通信地址：650205 昆明市北京路2238号 云南省农业科学院园艺作物研究所，Tel：0871-5892704，E-mail：。
  • 作者简介:

    王连润，女，1978年出生，云南鹤庆人，副研究员，硕士，主要从事果树栽培及育种工作。通信地址：650205 昆明市北京路2238号 云南省农业科学院园艺作物研究所，Tel：0871-5892704，E-mail：。

  • 基金资助:
    国家自然科学基金“云南猕猴桃属种质资源遗传多样性研究”(31960579); 创新引导与科技型企业培育计划“云南省绥江县水果产业科技特派团”(202304BI090021)

Genetic Relationship Analysis of Wild Actinidia Germplasm Resources in Yunnan Based on SSR

WANG Lianrun(), WAN Hong, TAO Pang, CHEN Xia, LI Kunming, SHA Yucang, DING Renzhan()   

  1. Horticultural Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205
  • Received:2023-02-21 Revised:2023-10-31 Published-:2024-02-05 Online:2024-01-29

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摘要：

为探讨云南猕猴桃属种质资源的亲缘关系，采用SSR分子标记方法对70份云南野生猕猴桃种质资源的亲缘关系进行分析。UPGMA聚类分析结果表明，70份猕猴桃种质资源在遗传相似系数0.17水平上可分为4个类群，类群Ⅰ共6份材料，包括滇东南麻栗坡的中越猕猴桃MLP001和MLP010、西畴的中越猕猴桃TSH-1和TSH-4，以及滇南屏边的中越猕猴桃PB001和PB005；类群Ⅱ共31份材料，主要包括滇东北的18份材料，滇西北的3份紫果猕猴桃TC-8、TC-1、TC-10，以及5份贡山猕猴桃CJ-11、CJ-13、CJ-7、CJ-3、CJ-2；类群Ⅲ共14份材料，主要包括滇东北的10份美味猕猴桃和中华猕猴桃系列材料，滇东的JZS-5、JZS-9及JZS-7，以及滇东南西畴的黄毛猕猴桃XPS-1；类群Ⅳ共19份材料，主要包括滇东北的16份材料，滇东南西畴的京梨猕猴桃XCFD-1及革叶猕猴桃XCFD-3，果实无被毛且果皮带斑点，果实较小，与其他类群具有较远的亲缘关系。SSR分子标记反映了云南猕猴桃属70份种质资源间的遗传亲缘关系，其中一些材料按种类、资源分布地及形态学性状特征形成明显有规律的聚类关系。

关键词: 野生猕猴桃, 亲缘关系, 云南, 种质资源, SSR分子标记

Abstract:

In order to explore the genetic relationship of Actinidia germplasm resources in Yunnan, the genetic relationship of 70 wild Actinidia germplasm resources in Yunnan was analyzed by SSR molecular marker method. The results of UPGMA cluster analysis showed that the 70 Actinidia germplasm resources could be divided into four groups at the genetic similarity coefficient level of 0.17. Group I consisted of 6 materials, including A. indochinensis MLP001 and MLP010 from Malipo in southeastern Yunnan, A. indochinensis TSH-1 and TSH-4 from Xichou in southeastern Yunnan, A. indochinensis PB001 and PB005 from Pingbian in southern Yunnan. Group II consisted of 31 materials, including 18 materials from northeastern Yunnan, 3 A. arguta var. purpurea TC-8, TC-1 and TC-10 from northwestern Yunnan, and 5 A. pilosula CJ-11, CJ-13, CJ-7, CJ-3 and CJ-2 from Gongshan. Group III consisted of 14 materials, 10 A. deliciosa and A. chinensis series from northeastern Yunnan, JZS-5, JZS-9 and JZS-7 from eastern Yunnan, and A. fulvicoma XPS-1 from Xichou in southeastern Yunnan. Group IV consisted of 19 materials, 16 from northeastern Yunnan, A. callosa var. henryi XCFD-1 from from Xichou in southeastern Yunnan, and A. rubricaulis var. coriacea XCFD-3 from Xichou in southeastern Yunnan. These fruits are hairless and smaller, and have a mottled skin. They have a distant relationship with other groups. The SSR molecular markers reflected the genetic relationship among the 70 germplasm resources of Actinidia in Yunnan, and some of them form obvious and regular clustering relationships according to species, resource distribution and morphological characteristics.

Key words: wild Actinidia, genetic relationship, Yunnan, germplasm resources, SSR molecular marker