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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (23): 148-152.doi: 10.11924/j.issn.1000-6850.casb2023-0765

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

一例猪源性金黄色葡萄球菌的分离鉴定与药物敏感性试验

张鑫1(), 赵东辉2, 李杰3, 贾伟娟4, 刘志林1, 贾泽林1, 崔佳宇1, 王学理1()   

  1. 1 内蒙古民族大学动物科技学院，内蒙古通辽 028000
    2 通辽市动物检疫技术服务中心，内蒙古通辽 028000
    3 通辽市库伦旗动物疫病预防控制中心，内蒙古通辽 028000
    4 通辽市农牧科学研究所，内蒙古通辽 028000
  • 收稿日期:2023-11-08 修回日期:2024-02-16 出版日期:2024-08-15 发布日期:2024-08-09
  • 通讯作者:
    王学理，男，1975年出生，内蒙古乌海人，教授，博士，主要从事兽医微生物学与免疫方面的研究。通信地址：028000 内蒙古通辽市科尔沁区西拉木伦大街996号 内蒙古民族大学动物科技学院，Tel：0475-15924529577，E-mail：。
  • 作者简介:

    张鑫，女，2000年出生，内蒙古乌兰察布人，硕士，研究方向：兽医微生物学与免疫。通信地址：028000 内蒙古通辽市科尔沁区西拉木伦大街996号 内蒙古民族大学动物科技学院，Tel：0475-15144845347，E-mail：。

  • 基金资助:
    内蒙古自然科学基金“牛源致病性蜡样芽胞杆菌溶血性肠毒素的提取及生物学活性分析”(2021LHMS03008); 内蒙古直属高校基本科研业务费多学科交叉研究项目“蒙东地区肉牛重大疫病流行病学调查及应对分析”(GXKY22003)

Isolation and Identification of A Porcine Staphylococcus aureus and Drug Sensitivity Test

ZHANG Xin1(), ZHAO Donghui2, LI Jie3, JIA Weijuan4, LIU Zhilin1, JIA Zelin1, CUI Jiayu1, WANG Xueli1()   

  1. 1 College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia 028000
    2 Tongliao Animal Quarantine Technology Service Center, Tongliao, Inner Mongolia 028000
    3 Tongliao Kulun Banner Animal Disease Prevention and Control Center, Tongliao, Inner, Mongolia 028000
    4 Tongliao Agricultural and Animal Husbandry Science Research Institute, Tongliao, Inner Mongolia 028000
  • Received:2023-11-08 Revised:2024-02-16 Published:2024-08-15 Online:2024-08-09

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摘要：

本研究旨在鉴定和分析锦州某猪场病死猪脏器中分离出的一株病原菌，以期为该细菌病的临床诊治提供科学依据。通过LB琼脂培养进行革兰氏染色，BP特殊培养基培养、药物敏感性试验、16S rDNA测序及小鼠致病性试验，并制作病理组织切片进行观察。由革兰氏染色镜检结果可知该菌为革兰氏阳性球菌；BP培养皿可见黑色菌落生长及菌落周边透明圈；药敏试验表明该菌对恩诺沙星、氧氟沙星、阿米卡星和卡那霉素非常敏感；经16S rDNA测序，该菌和金黄色葡萄球菌16S rDNA片段相似性高；镜检观察病理切片，可见各组织脏器有明显病理损害。该猪场致病菌经分离鉴定为金黄色葡萄球菌，对部分抗生素产生耐药，小鼠攻毒试验具有一定的致病性。

关键词: 猪, 金黄色葡萄球菌, 分离鉴定, 耐药性, 药物敏感性试验, 16S rDNA测序

Abstract:

This study aims to investigate a strain of pathogenic bacteria, isolated from the organs of dead pigs on a Jinzhou pig farm. The research encompasses bacterial isolation and identification, drug sensitivity tests, and clinical diagnosis and treatment implications. Gram staining was conducted using LB agar culture, followed by BP special medium culture. Drug sensitivity testing, 16S rDNA sequencing, and mouse pathogenicity testing were also performed. Pathological tissue sections were prepared for microscopic observation. Gram staining microscopy results indicated that the bacterium was Gram-positive cocci. The BP Petri dish revealed black colony growth with a transparent ring around it. Drug sensitivity tests demonstrated high sensitivity to enrofloxacin, ofloxacin, amikacin, and kanamycin. 16S rDNA sequencing revealed a high similarity between the 16S rDNA fragments of this bacterium and Staphylococcus aureus. Microscopic examination of pathological sections revealed significant pathological damage in various tissues and organs. The causative organism on this pig farm was identified as Staphylococcus aureus, which exhibited resistance to certain antibiotics and demonstrated some pathogenicity in the mouse attack test.

Key words: swine, Staphylococcus aureus, separation and identification, drug resistance, drug susceptibility test, 16S rDNA sequencing