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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (3): 107-113.doi: 10.11924/j.issn.1000-6850.casb2024-0125

• 生物科学 • 上一篇    下一篇

水蜡A病毒丁香分离物的鉴定与全基因组序列分析

王昀轩(), 刘丽, 孙春辉, 鲁书豪, 梁宇卿, 崔雯莉, 高佳仪, 李永强()   

  1. 北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室，北京 102206
  • 收稿日期:2024-02-27 修回日期:2024-05-15 出版日期:2025-01-25 发布日期:2025-01-20
  • 通讯作者:
    李永强，男，1985年出生，河南人，副教授，博士，主要从事植物病毒病害及其致病机理、植物病毒的鉴定、快速检测以及病毒与寄主植物的相互作用。通信地址：102206 北京农学院生物与资源环境学院，生资楼A324，Tel：010-80795024，E-mail：。
  • 作者简介:

    王昀轩，女，2000年出生，北京人，研究生，研究方向：植物病理方向。通信地址：102206 北京农学院生物与资源环境学院，生资楼A324，Tel：010-80795024，E-mail：。

  • 基金资助:
    北京市教委科研项目“利用siRNA序列组装技术鉴定葡萄新病毒”(KM201610020008)

Ligustrum Virus A Isolates from Syringa lindl: Identification and Whole Genome Sequence Analysis

WANG Yunxuan(), LIU Li, SUN Chunhui, LU Shuhao, LIANG Yuqing, CUI Wenli, GAO Jiayi, LI Yongqiang()   

  1. College of Bioscience and Resource Environment, Beijing University of Agriculture / Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Beijing 102206
  • Received:2024-02-27 Revised:2024-05-15 Published:2025-01-25 Online:2025-01-20

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摘要：

研究旨在明确侵染北京丁香(Syringa lindl)并引起褪绿、花叶、卷叶症状的病毒种类，以及其基因组分子特征和进化关系。利用小RNA深度测序和RT-PCR技术对病样进行检测分析。深度测序结果显示病样中检测到水蜡A病毒(ligustrum virus A，LVA)并获得该病毒序列全长。北京丁香分离物LVA-NXY的基因组序列全长8488 nt，北京丁香分离物LVA-HLG的基因组序列全长8478 nt。序列一致性分析表明，LVA-NXY、LVA-HLG基因组序列与中国沈阳市的东北连翘分离物LVA-DBLQ基因组序列相似度最高。未在已报道的7个LVA分离物中检测到重组事件。基于基因组序列的系统发育树显示LVA-NXY、LVA-HLG与LVA-DBLQ、沈阳紫丁香分离物LVA-DX共聚同一分支中，显示出较近的亲缘关系。本研究报道了侵染北京丁香的水蜡A病毒基因组的全长序列，探究了病毒基因组分子特征及进化关系，为该病毒遗传变异研究及丁香病毒病的防控提供理论依据。

关键词: 丁香, 水蜡A病毒, 小RNA, 基因组, 序列分析, 分子特征, 进化关系

Abstract:

To identify and characterize the candidate viruses infecting Beijing lilac (Syringa lindl) associated with the symptoms of leaf chlorosis, mosaic and leaf curling, small RNA deep sequencing and RT-PCR were conducted with the diseased leaf samples. Ligustrum virus A (LVA) was detected and the complete genome sequences of two isolates were obtained. The Beijing lilac isolate LVA-NXY is 8488 nt in length, while the isolate LVA-HLG is 8478 nt. Sequence similarity analysis showed that LVA-NXY and LVA-HLG were most similar to the LVA-DBLQ isolate from Forsythia suspensa in Shenyang, China. No recombination events were detected among the seven reported LVA isolates. The phylogenetic tree based on the genome sequences indicated that LVA-NXY and LVA-HLG clustered together with LVA-DBLQ and the Syringa vulgaris isolate LVA-DX in the same branch, showing a close phylogenetic relationship. This study reports the complete genome sequence of ligustrum virus A infecting Beijing lilac and explores the molecular characteristics and evolutionary relationships of the virus which provides a theoretical basis for the study of genetic variation and the control of lilac virus disease.

Key words: Syringa lindl, Ligustrum virus A, small RNA, genome, sequence analysis, molecular characteristics, phylogenetic relationship