珍稀药用植物金线莲研究现状与展望

doi:10.11924/j.issn.1000-6850.casb2023-0185

中国农学通报 ›› 2024, Vol. 40 ›› Issue (5): 16-26.doi: 10.11924/j.issn.1000-6850.casb2023-0185

珍稀药用植物金线莲研究现状与展望

张闻婷(), 梅瑜, 王继华()

广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室/广东省道地南药资源保护与利用工程中心，广州 510640

收稿日期:2023-03-13 修回日期:2023-08-17 出版日期:2024-02-01 发布日期:2024-02-01
通讯作者:
王继华，男，1979年出生，湖北荆门人，研究员，博士，研究方向为南药栽培与育种。通讯地址：510640 广东广州天河区金颖西二街18号广东省农业科学院作物研究所，Tel：020-87541262，E-mail：wangjihua@gdaas.cn。
作者简介:
张闻婷，女，1988年出生，浙江台州人，助理研究员，博士，研究方向为药用植物基因组学。通讯地址：510640 广东广州天河区金颖西二街18号广东省农业科学院作物研究所，Tel：020-87541262，E-mail：zhangwenting@gdaas.cn。
基金资助:
国家自然科学基金(32200301); 广州市科技计划项目(2023A04J0778); 广东省农科院优秀博士项目(R2021YJ-YB2008); 广东省农业科学院创新基金(202148); 广东省道地南药资源保护与利用工程技术研究中心开放课题(NYGC202203); 广东省科技计划项目(2023B1212060038)

Research Status and Prospect of Precious Medicinal Plant Anoectochilus roxburghii

ZHANG Wenting(), MEI Yu, WANG Jihua()

Crop Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of Crops Genetics and Improvement/Guangdong Provincial Engineering & Technology Research Center for Conservation and Utilization of the Genuine Southern Medicinal Resources, Guangzhou 510640

Received:2023-03-13 Revised:2023-08-17 Published-:2024-02-01 Online:2024-02-01

摘要/Abstract

摘要：

金线莲为兰科珍稀名贵药用和观赏植物，因富含多种药用和营养成分深受人们青睐，近年来的市场需求也在不断递增。为了促进金线莲的产业发展和基础研究，总结了前人在金线莲研究方面的进展，比较了金线莲属植物的种系差异，重点讨论了金线莲基因组学（结构基因组学/功能基因组学）以及环境（生物/非生物互作）响应方面的研究进展，分析了金线莲品质影响的主要因素为加工方式、栽培模式、品系，针对目前并未深入展开的种质资源概况、基因组学、环境互作、品质差异和胚胎繁殖等方面的研究进行了系统综述，并对目前存在的主要问题及如何解决提出相应的策略和展望，为大数据时代名贵药用植物的分子生物学研究提供可行性参考。

关键词: 金线莲, 基因组学, 环境响应, 品质影响, 胚胎繁殖

Abstract:

Anoectochilus roxburghii is a rare and precious medicinal and ornamental plant of the Orchidaceae. It is favored by people for the rich variety of medicinal and nutritional components, and its market demand has been increasing in recent years. In order to promote the industrial development and basic research of A. roxburghii, this paper summarizes previous studies on A. roxburghii research, briefly compares the phylogenetic differences of Anoectochilus, focus on the research results of genomics (structural genomics/functional genomics) and adversity (biotic/abiotic interaction) response, analyzes the main factors affecting the quality of A. roxburghii (processing methods, cultivation models, strains). This paper systematically reviews the studies on germplasm resources, genomics, environmental interaction, quality difference and embryo reproduction that have not been carried out in depth, and puts forward corresponding strategies and prospects for the main problems and the solutions. It provides a feasible reference for the molecular biology research of precious medicinal plants in the era of big data.

Key words: Anoectochilus roxburghii, genomics, stress response, quality effects, embryonic reproduction

张闻婷, 梅瑜, 王继华. 珍稀药用植物金线莲研究现状与展望[J]. 中国农学通报, 2024, 40(5): 16-26.

ZHANG Wenting, MEI Yu, WANG Jihua. Research Status and Prospect of Precious Medicinal Plant Anoectochilus roxburghii[J]. Chinese Agricultural Science Bulletin, 2024, 40(5): 16-26.

图/表 4

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编号	种名	主要栽培地	参考文献
1	Anoectochilus malipoensis	中国云南	[22]
2	Anoectochilus longilobus	中国云南	[23]
3	Anoectochilus elwesii	中国云南	[24]
4	Anoectochilus lylei	中国云南	[25]
5	Anoectochilus brevilabris	中国云南	[26]
6	Anoectochilus nandanensis	中国广西	[27-28]
7	Anoectochilus calcareus	中国广西	[27]
8	Anoectochilus zhejiangensis	中国浙江	[29]
9	Anoectochilus emeiensis	中国四川	[30]
10	Anoectochilus koshunensis	中国台湾	[31-32]
11	Anoectochilus formosanus	中国台湾	[33]
12	Anoectochilus roxburghii	中国福建	[34]
13	Anoectochilus xingrenensis	中国贵州	[35]
14	Anoectochilus annamensis	越南	[36-37]
15	Anoectochilus acalcaratus	越南	[38]
16	Anoectochilus calcareus	越南	[39]
17	Anoectochilus setaceus	越南，斯里兰卡	[40-41]
18	Anoectochilus sikkimensis	印度	[42]
19	Anoectochilus regalis	印度	[42]
20	Anoectochilus elatus	印度	[43]
21	Anoectochilus burmannicus	泰国	[44-45]
22	Anoectochilus sandvicensis	美国夏威夷	[46]

编号	种名	主要栽培地	参考文献
1	Anoectochilus malipoensis	中国云南	[22]
2	Anoectochilus longilobus	中国云南	[23]
3	Anoectochilus elwesii	中国云南	[24]
4	Anoectochilus lylei	中国云南	[25]
5	Anoectochilus brevilabris	中国云南	[26]
6	Anoectochilus nandanensis	中国广西	[27-28]
7	Anoectochilus calcareus	中国广西	[27]
8	Anoectochilus zhejiangensis	中国浙江	[29]
9	Anoectochilus emeiensis	中国四川	[30]
10	Anoectochilus koshunensis	中国台湾	[31-32]
11	Anoectochilus formosanus	中国台湾	[33]
12	Anoectochilus roxburghii	中国福建	[34]
13	Anoectochilus xingrenensis	中国贵州	[35]
14	Anoectochilus annamensis	越南	[36-37]
15	Anoectochilus acalcaratus	越南	[38]
16	Anoectochilus calcareus	越南	[39]
17	Anoectochilus setaceus	越南，斯里兰卡	[40-41]
18	Anoectochilus sikkimensis	印度	[42]
19	Anoectochilus regalis	印度	[42]
20	Anoectochilus elatus	印度	[43]
21	Anoectochilus burmannicus	泰国	[44-45]
22	Anoectochilus sandvicensis	美国夏威夷	[46]

珍稀药用植物金线莲研究现状与展望

Research Status and Prospect of Precious Medicinal Plant Anoectochilus roxburghii

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题目	综述内容	发表时间	参考文献
闽产金线莲产业化发展现状与对策研究	闽产金线莲产业发展现状、存在的问题、建议与对策	2022.10	[3]
金线莲的研究进展	金线莲种植技术现状、化学成分、质量标准、传统功效、现代药理、产业化瓶颈	2022.01	[4]
药用植物金线莲的研究进展	金线莲生物学特性、品种与特点、栽培技术、经济效益、产业市场	2021.11	[5]
西苑乡林下种植金线莲产业发展现状与对策	金线莲产业发展现状、发展意义、发展优势、存在问题、对策与建议	2021.07	[6]
金线莲保肝作用及作用机制研究进展	金线莲对四氯化碳(CCl4)肝损伤、酒精性肝病(ALD)、非酒精性脂肪性肝病(NAFLD)、自身免疫性肝炎(AIH)、慢性乙型肝炎(CHB)的保肝作用	2021.07	[7]
金线莲研究现状与保护措施	金线莲生物学特性、资源分布、药用研究、分子生物学、组织培养	2021.01	[8]
金线莲生药鉴定、活性成分影响因素及药理作用研究进展	金线莲的生药鉴定、活性成分影响因素、药理作用	2020.08	[9]
金线莲组织培养研究进展	金线莲人工繁殖、组培外植体选取和消毒、（基本、诱导、生根的）培养基选择	2020.02	[10]
金线莲组培技术现状及发展趋势	消毒剂的选择与使用、培养基选择、培养基添加物、光照、移栽条件、肥料等对金线莲组织培养技术的影响因素	2019.11	[11]
中药材金线莲药效成分分析与质量评价	金线莲中具有药理作用的化学成分、提取方法与质量分析	2019.10	[12]
金线莲研究进展	金线莲化学成分、组织培养外植体选取、培养基组分、生长的光照和温度条件、产品开发及产业化发展	2018.12	[13]
金线莲抗癌活性成分及抗癌作用机制的研究进展	金线莲抗癌活性成分及抗癌作用机制	2018.10	[14]
金线莲多糖提取及功能研究进展	金线莲多糖的提取方法和生物活性作用	2018.09	[15]
福建药用植物金线莲研究进展	金线莲的化学成分、提取分析、生物活性	2018.08	[16]
金线莲组织培养技术研究进展	金线莲外植体培养、组织培养体系、影响组培的重要因素	2018.05	[17]
金线莲的化学成分及生物活性研究进展	金线莲化学成分和生物活性	2018.04	[18]
金线莲活性成分和分子鉴定的研究进展	金线莲的生物学特性、化学成分和生理活性、分子鉴定、组织培养、人工栽培	2018.03	[19]
金线莲组织培养技术研究进展	金线莲外植体脱分化、芽诱导途径，植物原球茎诱导途径，芽苗的继代培养，壮苗与生根，成苗	2018.03	[20]

标记简称	标记全称	参考文献
SSR	简单重复序列(simple sequence repeat)	[61]
ISSR	内部简单重复序列(inter-simple sequence repeat)	[62-63]
AFLP	扩增片段长度多态性(amplified fragment length polymorphism)	[64]
SRAP	序列相关扩增多态性(sequence related amplified polymorphism)	[63]
SCoT	目标起始密码子多态性(start codon targeted polymorphism)	[65]
CDDP	保守DNA衍生多态性(conserved DNA-derived polymorphism)	[66]
DALP	直接扩增片段长度多态性(direct amplification of length polymorphism)	[67]
RAPD	DNA 随机扩增长度多态性(Random Aplified Polymorphism DNA)	[68]
SCAR	序列特征化扩增区域(sequence characterized amplified region)	[68]
CAPS	切割扩增多态性序列(cleaved amplified polymorphic sequence)	[69]

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