欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (20): 60-66.doi: 10.11924/j.issn.1000-6850.casb2024-0537

• 生物科学 • 上一篇    下一篇

外源褪黑素对盐碱胁迫下谷子萌发和幼苗抗氧化生理的影响

张梦媛1(), 王思玉2, 徐凤2, 刘一周1, 管延安1, 陈二影1, 黎飞飞1, 杨延兵1, 秦岭1()   

  1. 1 山东省农业科学院作物研究所/特色杂粮作物山东省工程研究中心，济南 250100
    2 新疆农业大学农学院，乌鲁木齐 830000
  • 收稿日期:2024-08-27 修回日期:2025-04-10 出版日期:2025-07-15 发布日期:2025-07-21
  • 通讯作者:
    秦岭，女，1977年出生，山东德州人，副研究员，博士，研究方向：作物遗传育种与抗逆机制研究。通信地址：250100 山东省济南市历城区工业北路23788号 山东省农业科学院，E-mail：。
  • 作者简介:

    张梦媛，女，1997年出生，河北石家庄人，研究生，研究方向：谷子抗逆资源研究。通信地址：050100 河北省石家庄市井陉矿区汇景家园8号楼6单元201，E-mail：。

  • 基金资助:
    国家重点研发计划项目“谷子高粱抗逆种质资源的精准鉴定”(2023YFD1202703-3); 国家现代农业产业技术体系项目“国家谷子高粱产业技术体系水分管理与节水栽培岗位”(CARS-06-13.5-A19); 山东省农业科学院农业科技创新工程“粮食作物突破性品种分子设计与应用”(CXGC2024A01); 济南市农业应用技术创新计划项目“小米糠高值化利用及产品开发研究”(CX202106)

Effects of Melatonin on Seed Germination and Antioxidant Physiology of Foxtail Millet Seedlings Under Saline-alkali Stress

ZHANG Mengyuan1(), WANG Siyu2, XU Feng2, LIU Yizhou1, GUAN Yan’an1, CHEN Erying1, LI Feifei1, YANG Yanbing1, QIN Ling1()   

  1. 1 Crop Research Institute, Shandong Academy of Agricultural Sciences/ Shandong Provinical Research Center for Featured Minor Crops, Jinan 250100
    2 College of Agronomy, Xinjiang Agricultural University, Urumqi 830000
  • Received:2024-08-27 Revised:2025-04-10 Published:2025-07-15 Online:2025-07-21

RichHTML

1

PDF (PC)

9

摘要：

本研究旨在探究外源褪黑素对盐碱胁迫下谷子萌发及幼苗抗氧化生理的影响。以谷子品种‘济谷22’为试验材料，分别采用浓度为0、100、200、300 μmol/L的褪黑素溶液进行浸种处理，测定不同处理在盐碱胁迫条件下的萌发指标。基于萌发试验结果，筛选出褪黑素浸种的最适浓度，并对谷子幼苗进行喷施处理。在苗期，运用100 mmol/L的混合盐碱溶液(NaCl:NaHCO3=4:1)模拟盐碱胁迫环境，测定谷子幼苗的株高、鲜重、干重，以及丙二醛(MDA)、可溶性糖(SS)含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性。研究结果显示，盐碱胁迫显著降低了谷子的发芽势、发芽率、发芽指数、根长、芽长、幼苗株高以及地上部物质的积累量，同时提高了SOD、POD、CAT活性以及MDA和SS的含量。施加不同浓度的褪黑素对盐碱胁迫下谷子的萌发和幼苗生长具有一定的缓解作用，其中 200 μmol/L的褪黑素溶液效果最为显著。喷施该最适浓度的褪黑素溶液能够显著提高抗氧化酶(SOD、POD、CAT)的活性以及SS含量，降低MDA含量。综上所述，盐碱胁迫会对谷子的萌发和幼苗生长产生抑制作用，而施加适宜浓度的褪黑素能够有效缓解盐碱胁迫对谷子造成的损伤，增强谷子的耐盐碱性。

关键词: 谷子, 盐碱胁迫, 褪黑素, 萌发期, 苗期，生理指标

Abstract:

The study aimed to explore the effects of exogenous melatonin on seed germination and antioxidant physiology of foxtail millet seedlings under saline-alkali stress. Using the foxtail millet variety ‘Jigu 22’ as the experimental material, seeds were soaked in melatonin solutions at concentrations of 0, 100, 200, and 300 μmol/L. Germination index under saline-alkali stress was measured, and based on the germination test results, the melatonin concentration for seed soaking was selected for leaf spraying application during the seedling stage. At the seedling stage, a mixed saline-alkali solution (100 mmol/L, NaCl:NaHCO3=4:1) was used to simulate saline-alkali stress. Seedling height, fresh weight, dry weight, malondialdehyde (MDA) and soluble sugar (SS) contents were measured, as well as the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). The results showed that saline-alkali stress significantly reduced germination vigor, germination rate, germination index, root length, shoot length, seedling height, and stems biomass accumulation in foxtail millet, while increasing the activities of SOD, POD, and CAT, as well as the contents of MDA and SS. Application of different concentrations of melatonin alleviated the adverse effects of saline-alkali stress on foxtail millet germination and seedling growth, among which the 200 μmol/L treatment exhibited the strongest protective effect. The application of the optimal melatonin concentration significantly enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and SS content while reducing MDA content. In summary, saline-alkali stress inhibits foxtail millet germination and seedling growth, whereas the application of an appropriate concentration of melatonin effectively mitigates the damage caused by saline-alkali stress and improves the salt-alkali tolerance of foxtail millet.

Key words: foxtail millet, saline-alkali stress, melatonin, germination stage, seedling stage, physiological index