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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (23): 1-9.doi: 10.11924/j.issn.1000-6850.casb2024-0675

• 农学·农业基础科学 •    

多年生稻苗期耐盐性评价

刘永秀1(), 赵颖1,2, 徐玲1, 袁圆1, 昂磊1, 罗静1, 杨涛1, 张清华1, 徐叶菊1, 张石来1,2()   

  1. 1 云南大学农学院，农业农村部多年生稻生物学与种质创新重点实验室，云南省多年生稻技术创新中心，昆明 650091
    2 西南联合研究生院，昆明 650092
  • 收稿日期:2024-11-08 修回日期:2025-07-11 出版日期:2025-08-19 发布日期:2025-08-19
  • 通讯作者:
    张石来，男，1985年出生，江西赣州人，研究员，博士，研究方向：多年生稻种质创新与遗传育种。通信地址：650504 云南省昆明市呈贡区东外环南路 云南大学农学院，E-mail：。
  • 作者简介:

    刘永秀，女，1998年出生，贵州遵义人，硕士研究生，研究方向：作物遗传育种。通信地址：650504 云南省昆明市呈贡区东外环南路 云南大学农学院，E-mail：。

  • 基金资助:
    云南省科技人才与平台计划“云南省刘巧泉专家工作站”(202405AF140110); 中青年学术和技术带头人后备人才项目(202305AC160015); 云南省基础研究计划项目“多年生稻种质创新与生物育种”(202501AV070007)

Evaluation of Salt Tolerance of Perennial Rice at Seedling Stage

LIU Yongxiu1(), ZHAO Ying1,2, XU Ling1, YUAN Yuan1, ANG Lei1, LUO Jing1, YANG Tao1, ZHANG Qinghua1, XU Yeju1, ZHANG Shilai1,2()   

  1. 1 Key Laboratory of Biology and Germplasm Innovation of Perennial Rice, Ministry of Agriculture and Rural Affairs / Yunnan Provincial Center of Perennial Rice Technology Innovation / School of Agriculture, Yunnan University, Kunming 650091
    2 Southwest United Graduate School, Kunming 650092
  • Received:2024-11-08 Revised:2025-07-11 Published:2025-08-19 Online:2025-08-19

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摘要：

本研究旨在明确多年生稻品种（系）‘PR23’、‘PR24’、‘PR25’、‘PR26’、‘PR101’、‘PR107’和‘PR109’的耐盐性，筛选出优异耐盐稻作新种质。试验采用浓度为0(CK组)、50、100、150、200 mmol/L NaCl溶液，对上述多年生稻品种（系）及母本‘RD23’进行盐胁迫处理，测定不同浓度下各品种（系）的生长指标（苗高、根长、鲜重、干重等）及耐盐系数，并通过主成分分析与隶属函数法综合评价其耐盐性能。结果表明：（1）当NaCl浓度达到150 mmol/L时，各品种（系）在盐害等级上出现了明显差异。在所有盐胁迫相关性状的表型中，除根长外，所有品种（系）在150 mmol/L NaCl处理下的指标均与CK组表现出显著差异(P < 0.05)，因此150 mmol/L NaCl被确定为多年生稻苗期最佳耐盐筛选浓度。（2）对150 mmol/L NaCl处理下各品种（系）耐盐性状的相对值进行主成分分析，计算得到隶属函数值，结合主成分方差贡献率权重进一步得出综合评价D值。结果显示，‘云大107(PR107)’在苗期表现出最强综合耐盐能力，‘PR101’在苗期的耐盐能力最弱，其他品种（系）的耐盐能力介于两者之间。本研究筛选出的优异耐盐稻作种质资源，不仅为水稻耐盐性遗传改良奠定基础，还为进一步开展水稻耐盐性机制研究提供重要数据支撑。

关键词: 耐盐性, 多年生稻, 苗期, NaCl胁迫, 主成分分析, 隶属函数法

Abstract:

This study aims to assess the salt tolerance of perennial rice varieties (lines) ‘PR23’, ‘PR24’, ‘PR25’, ‘PR26’, ‘PR101’, ‘PR107’, and ‘PR109’, and to screen out elite salt-tolerant germplasm. The experiment employed NaCl solutions at concentrations of 0, 50, 100, 150, and 200 mmol/L to conduct salinity stress treatments on perennial rice varieties (lines) and the parent ‘RD23’. The vitality parameters (seedling height, root length, fresh weight, dry weight, etc.) and salt tolerance coefficients of varieties were determined under different NaCl concentrations. Principal component analysis (PCA) and the membership function method were used to comprehensively evaluate the salt tolerance performance of the varieties (lines). The results showed that: (1) significant differences in salt damage levels among varieties (lines) were observed when NaCl concentration reached 150 mmol/L. Among all the phenotypic traits associated with salt stress, except for root length, all other traits under the 150 mmol/L NaCl treatment showed significant differences from the control group (P < 0.05), establishing this concentration as the optimal level for screening salt tolerance in perennial rice during the seedling stage. (2) PCA of the relative values of salt tolerance traits under the 150 mmol/L NaCl treatment generated membership function values. Combining with the principal component variance contribution rate weight, the comprehensive evaluation D value was obtained, showing that ‘Yun Da 107 (PR107) ’exhibited the strongest comprehensive salt tolerance ability at the seedling stage, while ‘PR101’ showed the weakest salt tolerance capacity. Other varieties (lines) had intermediate salt tolerance levels. This study provides high-quality germplasm resources for rice salt tolerance breeding and lays the foundation for future improvements in rice salt tolerance. This study not only identifies elite salt-tolerant rice germplasm resources through phenotypic and genotypic assessments, laying a foundation for genetic improvement of rice salt tolerance, but also provides critical data support for further research on salinity stress tolerance mechanisms.

Key words: salt tolerance, perennial rice, seedling stage, NaCl stress, principal component analysis (PCA), membership function method