欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (17): 23-33.doi: 10.11924/j.issn.1000-6850.casb2025-0747

• 生物科学 • 上一篇    下一篇

大豆GH3基因家族鉴定及表达模式分析

尹毛珠1(), 王月英1, 冯慧2, 王朝伟1, 高亮1, 周言虎1, 张亚楠3, 张玉坤1(), 刘兴舟1, 王路路1()   

  1. 1 宿州市农业科学院， 安徽宿州 234000
    2 中国农业科学院烟草研究所， 山东青岛 266000
    3 淮北师范大学生命科学学院， 安徽淮北 235000
  • 收稿日期:2025-09-05 修回日期:2026-01-11 出版日期:2026-09-15 发布日期:2026-09-09
  • 通讯作者:
    王路路，女，1984年出生，安徽固镇人，副研究员，主要从事大豆育种及高产栽培研究、绿色病虫害防治研究。通信地址：234000 安徽省宿州市埇桥区学府大道2156号 宿州市农业科学院，Tel：0557-2226818，E-mail：；
    张玉坤，男，1978年出生，安徽阜阳人，副研究员，主要从事小麦遗传育种及安徽省小麦试验汇总工作。通信地址：234000 安徽省宿州市埇桥区学府大道2156号 宿州市农业科学院，Tel：0557-2256829，E-mail：。
  • 作者简介:

    尹毛珠，女，1995年出生，安徽宿州人，助理研究员，研究方向：大豆品种选育与病虫害防治。通信地址：234000 安徽省宿州市埇桥区学府大道2156号 宿州市农业科学院，Tel：0557-2256829，E-mail：。

  • 基金资助:
    国家大豆产业技术体系建设专项(CARS-04-CES21); 国家自然科学基金“生物钟基因(SlitPer); 国家自然科学基金“生物钟基因(SlitCry2); 对斜纹夜蛾性信息素合成及释放节律的调控作用”(31970456); 安徽省重点研究与开发计划项目“大豆重大害虫点蜂缘蝽绿色防控关键技术研发与应用”(202104a06020035)

Identification and Expression Characterization of GH3 Gene Family of Soybean (Glycine max)

YIN Maozhu1(), WANG Yueying1, FENG Hui2, WANG Chaowei1, GAO Liang1, ZHOU Yanhu1, ZHANG Yanan3, ZHANG Yukun1(), LIU Xingzhou1, WANG Lulu1()   

  1. 1 Suzhou Academy of Agricultural Sciences, Suzhou, Anhui 234000
    2 Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266000
    3 College of Life Sciences, Huaibei Normal University, Huaibei, Anhui 235000
  • Received:2025-09-05 Revised:2026-01-11 Published:2026-09-15 Online:2026-09-09

RichHTML

12

PDF (PC)

27

摘要：

为了系统鉴定大豆GH3基因家族成员并揭示其进化特征及潜在功能。以‘中黄13’基因组为试材，采用生物信息学方法鉴定GH3家族成员，系统分析其理化性质、染色体定位、共线性关系、系统进化、基因结构、保守基序、二级结构、启动子元件及表达模式。共鉴定到35个GH3成员，它们分布于16条染色体上，编码的蛋白大部分为酸性亲水性蛋白。种内及大豆不同品种种间的共线性分析表明，GH3基因家族在进化中具有高度保守性。系统进化分析将GH3蛋白分为3个亚群，同一亚群内的基因结构与保守基序高度相似，提示它们可能协同发挥作用。蛋白的二级结构以α-螺旋(40.77%)和随机卷曲(40.81%)为主，延伸链和β-折叠分别占14.16%和4.25%。启动子区域分析检测到与逆境胁迫以及生长发育相关的调控元件。表达谱分析显示，SoyZH13_02G116300与SoyZH13_03G235000在多个发育阶段均有表达。本研究为阐明GH3基因在大豆生长发育中的调控作用提供了理论基础，并为理解多种激素在大豆生长发育中的协同作用提供了新的视角。

关键词: 大豆, GH3基因家族, 全基因组鉴定, 生长素, 表达模式, 基因结构, 理化性质, 逆境胁迫

Abstract:

To systematically identify the GH3 gene family members in soybean (Glycine max) and reveal their evolutionary characteristics and potential functions, this study utilized the genome of ‘Zhonghuang 13’ as the experimental material. Bioinformatics methods were employed to identify members of the GH3 family, and their physicochemical properties, chromosomal localization, collinearity relationships, phylogenetic evolution, gene structure, conserved motifs, secondary structure, promoter elements, and expression patterns were systematically analyzed. A total of 35 GH3 members were identified, distributed across 16 chromosomes, most of the encoded proteins were acidic and hydrophilic proteins. Collinearity analysis within the soybean genome and among different soybean varieties revealed a high degree of conservation in the evolution of this family. Phylogenetic analysis classified the proteins into three subfamilies, with members of the same subfamily showing high similarity in gene structure and conserved motifs, suggesting that they may play a synergistic role. The secondary structure was predominantly composed of alpha helices (40.77%) and random coils (40.81%), with extended strands and beta sheets accounting for 14.16% and 4.25%, respectively. Promoter regions contained elements related to stress responses and growth and development. Expression analysis showed that SoyZH13_02G116300 and SoyZH13_03G235000 were expressed at multiple developmental stages. This study provides a theoretical foundation for elucidating the roles of GH3 genes in soybean growth and development and offers new insights into the synergistic effects of multiple hormones on soybean growth and development.

Key words: soybean, GH3 gene family, genome-wide identification, auxin, expression pattern, gene structure, physicochemical properties, abiotis stress

中图分类号: