白菜内生细菌的分离及其促生特性研究

doi:10.11924/j.issn.1000-6850.casb2026-0365

中国农学通报 ›› 2026, Vol. 42 ›› Issue (21): 37-45.doi: 10.11924/j.issn.1000-6850.casb2026-0365

• 农业微生物组与绿色发展专题 • 上一篇下一篇

白菜内生细菌的分离及其促生特性研究

刘雨萌(), 付海燕, 刘春光, 宋新宇, 杨峰山()

黑龙江大学，生命科学学院，农业微生物技术教育部工程研究中心，黑龙江省寒地生态修复与资源利用重点实验室，黑龙江省普通高校分子生物学重点实验室，哈尔滨 150080

收稿日期:2026-05-07 修回日期:2026-07-15 出版日期:2026-11-10 发布日期:2026-11-10
通讯作者:
杨峰山，男，1973年出生，山东文登人，教授，博士，主要从事作物害虫防治和农田土壤修复方面的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，Tel：0451-86608586，E-mail：yangfengshan@hlju.edu.cn。
作者简介:
刘雨萌，女，1999年出生，辽宁凤城人，在读硕士，研究方向：修复生态学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，Tel：18088701628，E-mail：1258492870@qq.com。
基金资助:
国家重点研发计划“蔬菜重大病虫害演替规律与全程绿色防控技术体系集成示范”(2022YFD1401200)

Isolation and Growth-promoting Characteristics of Endophytic Bacteria from Chinese Cabbage

LIU Yumeng(), FU Haiyan, LIU Chunguang, SONG Xinyu, YANG Fengshan()

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/ Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/ Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2026-05-07 Revised:2026-07-15 Published:2026-11-10 Online:2026-11-10

摘要/Abstract

摘要：

研究旨在挖掘白菜内生细菌的促生潜力，为开发高效植物促生菌剂提供优良菌种资源。以20个不同品种的白菜为试材，采用平板涂布法分离纯化内生细菌，利用CAS平板法、PKO培养基检测法、Salkowski 比色法，评估各菌株的体外促生特性，筛选出2株促生效果最佳的菌株，通过16S rDNA序列鉴定，并验证其对种子萌发和植株生长的促进效果。结果表明：从白菜根部和叶片共分离得到158株内生细菌，筛选出2株具有较好促生功能的菌株Br11TL-3、Br18RR-3，鉴定其分别为桔黄假单胞菌(Pseudomonas chlororaphis subsp. Aurantiaca)和砷氏土壤杆菌(Agrobacterium arsenijevicii)。2株菌对白菜种子的萌发均有促进作用。其中，菌株Br11TL-3处理组的白菜鲜重增幅最大，且显著提高植株根冠比，表明其具有促进整体生长和根系发育的潜力；菌株Br18RR-3对白菜植株叶绿素含量提升效果最优，达到2.21 mg/g，预示其可增强植株光合能力。研究表明，2株内生细菌均对白菜的生长起到促进作用，菌株Br11TL-3的综合促生效果更佳，具有良好的应用前景，为绿色高效的白菜种植技术提供了优质菌种资源和理论基础。

关键词: 内生细菌, 白菜, 分离培养, 促生作用, 吲哚乙酸(IAA), 固氮作用, 解磷作用, 种子萌发

Abstract:

The aim of this study is to explore the growth-promoting potential of endophytic bacteria in Chinese cabbage, and to provide excellent strain resources for the development of efficient plant growth-promoting agents. In this study, 20 varieties of Chinese cabbage were used as experimental materials. Endophytic bacteria were isolated and purified using the plate streaking method. Their plant growth-promoting traits were assessed using the CAS assay, the PKO medium assay, and the Salkowski colorimetric method. Two endophytic bacterial strains exhibiting the strongest promoting effects were selected for 16S rDNA sequence analysis, and their effects on seed germination and plant growth were further validated. The results showed that 158 endophytic bacterial were isolated from two tissue parts of Chinese cabbage. Two strains with superior plant growth-promoting capabilities, Br11TL-3 and Br18RR-3, were selected. They were identified as Agrobacterium arsenijevicii and Pseudomonas chlororaphis subsp. aurantiaca, respectively. Both strains enhanced Chinese cabbage seed germination; the Br11TL-3-treated group showed the greatest increase in fresh weight and significantly improved root-to-shoot ratio; the Br18RR-3 strain produced the most pronounced improvement in chlorophyll content (2.21 mg/g). This study demonstrates that both strains promote Chinese cabbage growth, with Br11TL-3 showing particularly strong promotion and promising potential for agricultural application.

Key words: endophytic bacteria, cabbage, isolation culture, growth promoting effect, indole acetic acid(IAA), nitrogen fixation, phosphorus solubilization, seed germination

中图分类号:

S634.1

刘雨萌, 付海燕, 刘春光, 宋新宇, 杨峰山. 白菜内生细菌的分离及其促生特性研究[J]. 中国农学通报, 2026, 42(21): 37-45.

LIU Yumeng, FU Haiyan, LIU Chunguang, SONG Xinyu, YANG Fengshan. Isolation and Growth-promoting Characteristics of Endophytic Bacteria from Chinese Cabbage[J]. Chinese Agricultural Science Bulletin, 2026, 42(21): 37-45.

图/表 12

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菌株代号	产IAA量/(mg/L)	菌株代号	产IAA量/(mg/L)	菌株代号	产IAA量/(mg/L)
Br13RR-1	4.33±0.22a	Br11RR-3	3.56±0.01a	Br11TL-4	6.11±0.61a
Br10RL-3	8.82±0.60b	Br6RL-1	7.10±0.26b	Br13TL-3	8.95±0.78b
Br10RL-2	5.64±0.70a	Br18RR-3	11.79±1.71c	Br15RL-1	6.34±1.70a
Br12RR-3	9.62±1.73b	Br15TL-2	12.54±0.74c	Br20RR-1	4.93±0.36a
Br4RR-3	5.28±1.08a	Br14TR-2	10.54±0.06c	Br11TL-3	14.56±0.05d
Br1TL-1	13.43±0.57d	Br6RR-1	3.55±0.33a	Br7TL-2	6.13±1.38a

菌株代号	产IAA量/(mg/L)	菌株代号	产IAA量/(mg/L)	菌株代号	产IAA量/(mg/L)
Br13RR-1	4.33±0.22a	Br11RR-3	3.56±0.01a	Br11TL-4	6.11±0.61a
Br10RL-3	8.82±0.60b	Br6RL-1	7.10±0.26b	Br13TL-3	8.95±0.78b
Br10RL-2	5.64±0.70a	Br18RR-3	11.79±1.71c	Br15RL-1	6.34±1.70a
Br12RR-3	9.62±1.73b	Br15TL-2	12.54±0.74c	Br20RR-1	4.93±0.36a
Br4RR-3	5.28±1.08a	Br14TR-2	10.54±0.06c	Br11TL-3	14.56±0.05d
Br1TL-1	13.43±0.57d	Br6RR-1	3.55±0.33a	Br7TL-2	6.13±1.38a

菌株代号	固氮	产铁载体	产ACC脱氨酶	菌株代号	固氮	产铁载体	产ACC脱氨酶
Br10RL-2	+	+	+	Br7TL-2	-	-	+
Br13RR-1	-	-	-	Br15RL-1	-	+	+
Br2RR-1	-	-	-	Br6RL-1	-	-	+
Br15TR-2	-	-	+	Br1TL-1	+	+	+
Br14TR-2	+	-	-	Br20RR-1	-	-	+
Br12RL-3	-	-	+	Br15RR-4	-	-	-
Br6RR-1	+	+	-	Br18RR-3	+	+	+
Br10RL-3	-	+	+	Br13TL-3	+	-	+
Br11TL-3	+	+	+	Br12RR-3	-	-	-
Br11RR-3	-	-	-	Br4RR-3	-	-	-

菌株代号	固氮	产铁载体	产ACC脱氨酶	菌株代号	固氮	产铁载体	产ACC脱氨酶
Br10RL-2	+	+	+	Br7TL-2	-	-	+
Br13RR-1	-	-	-	Br15RL-1	-	+	+
Br2RR-1	-	-	-	Br6RL-1	-	-	+
Br15TR-2	-	-	+	Br1TL-1	+	+	+
Br14TR-2	+	-	-	Br20RR-1	-	-	+
Br12RL-3	-	-	+	Br15RR-4	-	-	-
Br6RR-1	+	+	-	Br18RR-3	+	+	+
Br10RL-3	-	+	+	Br13TL-3	+	-	+
Br11TL-3	+	+	+	Br12RR-3	-	-	-
Br11RR-3	-	-	-	Br4RR-3	-	-	-

生理生化反应	Br11TL-3	Br18RR-3	生理生化反应	Br11TL-3	Br18RR-3
硝酸盐还原	+	+	V-P试验	-	-
氧化酶	+	+	葡萄糖分解	+	+
明胶液化	-	+	接触酶	+	+
吲哚试验	-	-	柠檬酸盐利用	+	-

白菜内生细菌的分离及其促生特性研究

Isolation and Growth-promoting Characteristics of Endophytic Bacteria from Chinese Cabbage

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菌株代号	菌液浓度
	1.0×10⁸ CFU/mL		5×10⁷ CFU/mL		2.5×10⁷ CFU/mL
	发芽率	发芽势	发芽率	发芽势	发芽率	发芽势
CK(LB)	93.33%	73.33%	93.33%	73.33%	93.33%	73.33%
Br11TL-3	96.67%	83.33%	96.67%	90.00%	100.00%	93.33%
Br18RR-3	96.67%	86.67%	100.00%	90.00%	100.00%	93.33%

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