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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (21): 23-36.doi: 10.11924/j.issn.1000-6850.casb2025-1005

• 农业微生物组与绿色发展专题 • 上一篇    下一篇

接种丛枝菌根真菌(AMF)对玉米纹枯病抗性的影响

董成艳1(), 李正楠1, 邱炜铧1, 余静1, 陈一苇1, 张思颖1, 李小林2, 谭静1()   

  1. 1 云南大学农学院， 昆明 650500
    2 云南省农业科学院粮食作物研究所， 昆明 650205
  • 收稿日期:2025-12-21 修回日期:2026-03-20 出版日期:2026-11-10 发布日期:2026-11-10
  • 通讯作者:
    谭静，女，1975年出生，新疆奎屯人，研究员，硕士，研究方向：玉米优质抗逆遗传与改良。通信地址：650500 云南省昆明市呈贡区大学城东外环南路 云南大学农学院，Tel：0871-65031539，E-mail：。
  • 作者简介:

    董成艳，女，2001年出生，云南楚雄人，在读硕士，研究方向：玉米抗逆性遗传与改良。通信地址：650500 云南省昆明市呈贡区大学城东外环南路 云南大学农学院，Tel：0871-65031539，E-mail：。

  • 基金资助:
    云南大学研究生实践创新项目“玉米拟轮枝镰孢穗腐病抗性候选基因ZmOTUD12与ZmJAZ10c的功能验证与分子标记开发”(ZC-252511707); 云南省重点研发计划“云南高原粮经作物协同种植关键技术集成”(202503AS090005)

Effect of Arbuscular Mycorrhizal Fungi (AMF) Inoculation on Resistance to Banded Leaf and Sheath Blight Disease of Maize

DONG Chengyan1(), LI Zhengnan1, QIU Weihua1, YU Jing1, CHEN Yiwei1, ZHANG Siying1, LI Xiaolin2, TAN Jing1()   

  1. 1 School of Agriculture, Yunnan University, Kunming 650500
    2 Institute of Food Crops Research, Yunnan Academy of Agricultural Sciences, Kunming 650205
  • Received:2025-12-21 Revised:2026-03-20 Published:2026-11-10 Online:2026-11-10

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摘要：

本研究探讨了接种丛枝菌根真菌(Arbuscular Mycorrhizal Fungi，AMF)对玉米纹枯病抗性的影响，旨在为玉米纹枯病的绿色防控及稳产高产提供依据。以玉米感病代表自交系‘B73’为试材，设置单接种纹枯病主要致病菌立枯丝核菌(Rhizoctonia solani Kuhn) (R)、单接种AMF (A)、双接种(AR)和不接种(CK)共4个处理，分析不同处理下玉米的表型、理化指标与根际土壤微生物群落。结果表明：接种AMF显著促进了玉米生长，在病原菌胁迫下，AR处理的地上部和地下部干重分别较未接种R处理提高了87.11%和51.17%；接种AMF也显著抑制了纹枯病的扩展：接种后3 d和5 d，AR处理的病斑长度较未接种R处理分别降低了55.39%和49.24%；接种AMF和病原菌都能显著提高玉米叶鞘和根系中茉莉酸(JA)、水杨酸(SA)的含量，以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)的活性，双接种AR处理进一步提升了上述指标；接种AMF显著提高了玉米根际土壤细菌群落的多样性，深刻改变了群落组成和功能，其中拟杆菌门(Bacteroidota)、放线菌门(Actinobacteriota)和酸杆菌门(Acidobacteriota)等有益菌群的丰度显著增加。综上所述，AMF通过促进玉米生长、改善相关理化指标和优化根际土壤微生物群落等多重机制协同作用，增强了玉米对纹枯病的抗性。本研究结果为开发基于AMF的玉米纹枯病绿色生物防治策略提供了理论依据。

关键词: 玉米, 纹枯病, 丛枝菌根真菌(AMF), 立枯丝核菌(Rhizoctonia solani Kuhn), 理化指标, 根际微生物群落

Abstract:

This study aims to explore the impact of inoculating arbuscular mycorrhizal fungi (AMF) on the resistance of maize to banded leaf and sheath blight (BLSB), providing a basis for scientific prevention and control of BLSB and ensuring stable and high yield of maize. Taking the susceptible maize inbred line ‘B73’ as the test material, four treatments were set up, including single inoculation with the main pathogen of BLSB Rhizoctonia solani Kuhn (R), single inoculation with AMF (A), double inoculation (AR), and no inoculation (CK). The phenotype, physicochemical indicators, and rhizosphere soil microbial community of maize under different treatments were analyzed. The results showed that inoculating with AMF significantly promoted the growth of maize; under pathogen stress, the dry weight of the aboveground and underground parts in the AR treatment increased by 87.11% and 51.17% respectively, compared to the non-inoculated R treatment. Inoculating with AMF also significantly inhibited the spread of BLSB, the lesion length in the AR treatment decreased by 55.39% and 49.24% at 3 days and 5 days after inoculation respectively, compared to the non-inoculated R treatment. Both inoculation with AMF and pathogenic bacteria could significantly increase the content of JA and SA, as well as the activities of SOD, POD, PAL, and PPO in maize leaf sheaths and roots; double inoculation further enhanced these levels. Inoculating with AMF significantly enhanced the diversity of rhizosphere soil bacterial communities, leading to notable changes in community composition and function. The abundance of beneficial bacterial groups such as Bacteroidota, Actinobacteriota, and Acidobacteriota increased significantly. In summary, inoculating with AMF enhanced the resistance of maize to BLSB by promoting maize growth, improving relevant physicochemical indicators, and optimizing the rhizosphere soil microbial community. The research results can provide theoretical basis for developing AMF-based biological control strategies against maize BLSB.

Key words: maize, banded leaf and sheath blight (BLSB), arbuscular mycorrhizal fungi (AMF), Rhizoctonia solani Kuhn, physicochemical indicators, rhizosphere microbial community

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