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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (8): 121-126.

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

水稻不同品种蛋白质亚基百分含量的差异性研究

王继馨   

  • 收稿日期:2009-01-16 修回日期:2009-02-11 出版日期:2009-04-20 发布日期:2009-04-20

Studies on the difference of protein subunit’s structure in the different varieties of rice

Wang Ji-Xin   

  • Received:2009-01-16 Revised:2009-02-11 Online:2009-04-20 Published:2009-04-20

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摘要： 摘 要：主要通过SDS-PAGE电泳检测方法，探讨水稻谷蛋白PB-II亚基和醇溶蛋白PB-I亚基含量的差异。试验结果表明，不同水稻品种的蛋白质亚基含量不同。龙粳8号的醇溶蛋白亚基含量为11.53%；富士光和松99-135的醇溶蛋白亚基含量为13.39%和13.61%，居同一水平，其谷蛋白亚基含量较高为72.87%和69.52%，是富含谷蛋白的PB-II蛋白质体；绥粳3号的醇溶蛋白亚基含量相对较高为15.25%。对水稻品种谱带进行13kDa单一亚基分析，绥粳3号和籼粳交的特优21在单一亚基分析中，其13kDa醇溶蛋白亚基含量明显高于其它几个品种。不同品种的13kDa醇溶蛋白亚基含量存在差异。

关键词: 陆地棉, 陆地棉, 愈伤组织, 体细胞胚, 生化机制

Abstract: Abstract:The difference of protein PB-II subunit and gliadin PB - I subunit’s content in the rice through SDS-PAGE electrophoresis detection method was discussed. The results showed that different varieties of rice have the different protein subunits amount, for example existed prolamine amount 11.53% only in Longjing 8 and content prolamine amount 13.39% and 13.61% in 99-135 light and the Fujitsu-Zein subunit, ranking the same level, but it’s rich glutetnin amount in protein-protein PB-II for 72.87% and 69.52 %,; Sui Japonica No. 3 prolamine amount content of 15.25% prolamine amount subunit relatively high. According to a single13kDa subunit analysis of the rice’s species, the 13kDa prolamine amount subunit’s content was higher than that of several other varieties obviously in the 3rd Sui Japonica and Indica and Japonica intercourse Teyou 21. In other words, the content of 13kDa prolamine amount subunit is different in the different species.

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