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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (1): 169-172.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

重瓣长寿花叶片组织培养及植株再生的研究

段鹏慧 李兴泽   

  • 收稿日期:2009-09-01 修回日期:2009-10-13 出版日期:2010-01-05 发布日期:2010-01-05
  • 基金资助:

    山西林业职业技术学院科研基金

Tissue Culture and plantlet regeneration from leaves of Kalanchoe blossfeldiana

  • Received:2009-09-01 Revised:2009-10-13 Online:2010-01-05 Published:2010-01-05

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摘要：

摘要:以重瓣长寿花幼嫩叶片为试材，筛选各个培养阶段的培养基配方，研究重瓣长寿花的快速繁殖技术。结果表明: 0.1％升汞灭菌4min，外植体污染率为12.5％，死亡率2.5%，效果最佳；愈伤组织诱导培养基以MS+6-BA1.0 mg／L+NAA0.1～0.3 mg／L最佳，不定芽分化和增殖的最适培养基为MS+6-BA0.5 mg／L+NAA0.1 mg／L，生根培养基用1/2MS+NAA0.5mg／L最好，试管苗移栽成活率达99％。

关键词: 茶树, 茶树, 农药残留, 抗性机制

Abstract:

Abstract：The young leaves culture and the rapid propagation of Kalanchoe blossfeldiana were carried out and media for various culture stages were selected. The experimental results indicated that explant sterilized in 0.1％ aqueous mercuric chloride for 4 min has best effect,with the contamination rate 12.5% , and the death rate 2.5%. The best inducement culture medium is MS+6-BA1.0 mg／L+NAA0.1～0.3 mg／L. The suitable culture medium for bud differentiatio and proliferatio is 1/2MS + 6-BA1.0 mg／L + NAA0.1 mg／L. The plantlets are transplanted into nutrient matrix with 99％ survival ratio.