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中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 182-185.

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

海盐苦卤对番茄早疫病菌的抑制及其对番茄叶片防御酶系的影响

秦培文 宋文东 李晓菲 胡世伟   

  • 收稿日期:2010-12-17 修回日期:2011-02-14 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    广东省科技厅广东省社会发展领域重点项目“废弃浓盐水绿色利用技术研究”

The Inhibition of Sea Brine on Alternaria solani and its Effects on Defense Enzymes of Tomato Leaves

  • Received:2010-12-17 Revised:2011-02-14 Online:2011-07-05 Published:2011-07-05

摘要:

采用菌丝生长速率法测定了海盐苦卤对番茄早疫病菌的抑制,通过测定PAL、CAT和PPO的变化,研究了海盐苦卤对番茄叶片防御酶系(苯丙氨酸解氨酶PAL、过氧化氢酶CAT和多酚氧化酶PPO)的影响。结果表明:随着海盐苦卤浓度的降低,其对番茄早疫病菌作用表现为:抑制—促进—抑制,并在浓度为0.0050%具有最佳抑制效果,抑制率为42.3%;此外,浓度为0.050%的海盐苦卤处理能够提高番茄叶片PAL、CAT和PPO的活性。

关键词: 筛选, 筛选

Abstract:

In this paper, the inhibition of sea brine on Alternaria solani was measured by adopting the method of hypha growth rate and defense enzymes in tomato leaves were studied by using determination of PAL, CAT and PPO. The results showed that the inhibition of sea brine on hypha of Alternaria solani was inhibition-promotion-inhibition according to the concentration decrease of sea brine. There was a best inhibition rate (42.3%) at the concentration was 0.0050%. In addition, 0.050% sea brine could enhance the activity of PAL, CAT and PPO in tomato leaves.