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中国农学通报 ›› 2011, Vol. 27 ›› Issue (2): 400-404.

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亚硝酸钠对果蝇寿命和生育力的影响及维生素C对其拮抗作用研究

张书玲 董丽军 王瑞强   

  • 收稿日期:2010-06-24 修回日期:2010-07-30 出版日期:2011-01-14 发布日期:2011-01-14
  • 基金资助:

    保定市科学技术研究与发展指导计划项目

The Effect of Sodium Nitrite on Life-span and Reproductive Capacity of Drosophila melanogaster and Antagonism of Vc on Sodium Nitrite

  • Received:2010-06-24 Revised:2010-07-30 Online:2011-01-14 Published:2011-01-14

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摘要：

以黑腹果蝇(Drosophila melanogaster)为试验动物, 研究了亚硝酸钠对果蝇寿命和生育力的影响及维生素C（Vc）对其拮抗作用。结果表明：培养基中添加亚硝酸钠可以显著缩短果蝇的平均寿命、最短和最长平均寿命。与对照组相比，除30mg/mL亚硝酸钠处理组的雄果蝇差异不显著外，其它实验组果蝇平均寿命均显著或极显著缩短（P〈0.05或P〈0.01）。果蝇的生育力也随着亚硝酸钠浓度的升高而降低，各实验组与对照相比差异均达到极显著水平（P〈0.01）。随后通过在添加480mg/mL亚硝酸钠的培养基中加入不同浓度Vc，结果发现，除雄果蝇的0.13%实验组与对照差异不显著外，雌、雄果蝇的平均寿命随Vc浓度的提高而显著或极显著延长（P〈0.05或P〈0.01）。除0.13%实验组与对照差异不显著外，果蝇生育力其它两组均极显著高于对照组（P〈0.01）。

关键词: POD, POD, CAT, MDA

Abstract:

Abstract: In this study, Drosophila melanogaster was used as the experimental animal to study the effects of sodium nitrite on life span, reproductive capacity and antagonistic effects of Vc on sodium nitrite. The results showed that the lifespan, average longest and average shortest life span was shortened in sodium nitrite. Compared with the control group, the average life span of D.melanogaster was shortened in sodium nitrite significantly(P〈0.05 or P〈0.01) except for 30mg/ml sodium nitrite experimental group. The reproductive capacity of D.melanogaster was shortened in sodium nitrite significantly (P〈0.01). When the concentration of Vc were 0.13%, 0.40% and 1.20% in the 480mg/ml sodium nitrite Medium, with the concentration of Vc increased, the average life span of D.melanogaster was prolonged significantly(P〈0.05 or P〈0.01) except for 30mg/ml sodium nitrite experimental group of male D.melanogaster. The reproductive capacity of D.melanogaster was prolonged significantly (P〈0.01 except for 30mg/ml sodium nitrite experimental group).