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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (26): 39-42.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

糖皮质激素受体（GR）基因内含子7长度多态性与猪脂肪性状相关研究

魏麟 刘胜贵 黎晓英 胡朝暾 向孙军 姚元枝 陈斌 刘鹏   

  • 收稿日期:2011-06-13 修回日期:2011-06-28 出版日期:2011-10-15 发布日期:2011-10-15
  • 基金资助:

    现代农业产业技术体系建设专项资金

Associations between length polymorphism in intron 7 of GR gene and fat traits of pigs

  • Received:2011-06-13 Revised:2011-06-28 Online:2011-10-15 Published:2011-10-15

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摘要：

应用特异PCR技术检测了猪糖皮质激素受体（GR）基因内含子7长度多态性，并分析其与脂肪性状的相关性。结果表明：凉伞猪、龙潭猪群体中GR基因内含子7长度多态性发现有A和B两个等位基因存在，该两种等位基因A/B频率在两个群体中分别为0.2286/0.7714和0.2008/0.7992。B等位基因是群体中的优势等位基因，该基因座在两群体中处于Hardy-Weinberg平衡状态。建立混合模型方程组分析该长度多态对猪脂肪性状的效应，结果显示，猪GR基因内含子7长度多态的不同基因型与肌内脂肪、肩部膘厚、腰部膘厚、6～7肋膘厚、板油质量等性状显著相关（P<0.05）。表明猪GR基因内含子7长度多态对猪脂肪沉积有一定的影响。

关键词: 生物农药, 生物农药, 万寿菊, 厚朴, 番茄叶霉病菌, 乳油

Abstract:

The length polymorphism of intron 7 in glucocorticoid receptor (GR) gene was detected by PCR. A length polymorphic segment in intron 7 is common in all detected groups. Alleles detected in the sequence were allele A and B with frequencies of 0.2286/0.7714 and 0.2008/0.7992 in the two populations, respectively. This polymorphic segment of GR gene was at Hardy-Weinberg equilibrium (P＞0.05) in the two populations, respectively. The effects of the length polymorphic segment on fat traits in pigs were estimated using a mixed model．Significant associations were found between different genotypes of intron 7 in glucocorticoid receptor with intamuscular fat, backfat thickness of shoulder, waist, 6-7 ribs and the leaf fat weight (P＜0.05). which indicated that the length polymorphism had certain effect on porcine fat storage.