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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (9): 184-190.

所属专题： 资源与环境

• 农学 农业基础科学 • 上一篇    下一篇

山东半岛地区土壤中木霉菌资源分类研究

张红 颜艳伟 咸洪泉   

  • 收稿日期:2011-01-17 修回日期:2011-03-05 出版日期:2011-04-25 发布日期:2011-04-25

The Research of Classification of Tichoderma Species in Shandong Peninsula

  • Received:2011-01-17 Revised:2011-03-05 Online:2011-04-25 Published:2011-04-25

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摘要：

为明确山东半岛地区的木霉菌资源，从半岛地区采集的59份土壤样品中共分离纯化出木霉菌株127株，采用形态学及ITS/5.8S测序分析，对这些木霉进行了分类鉴定。结果表明形态学与ITS鉴定结果一致，共鉴定出7个木霉集合种：哈茨木霉、棘孢木霉、深绿木霉、黄绿木霉、粘绿木霉、橘绿木霉、长枝木霉。深绿木霉和哈茨木霉出现频率最高，分别是26.0%、25.2%，是土壤中的优势种群。

关键词: 花椰菜, 花椰菜, 随机扩增多态性DNA, 遗传多样性

Abstract:

In order to understand the resouce of Tichoderma species in Shandong Peninsula, one hundred and twenty-seven isolates of Trichoderma spp. were isolated form 59 soil samples that collected in Shandong Peninsula. The morphological characteristics and ITS analysis were used to identify the Trichoderma spp. strains. And the result of ITS analysis was coincident with the morphological characteristics. The result showed that the strains belonged to Trichoderma harzianum, Trichoderma atrotiviride, Trichoderma asperellum, Trichoderma virens, Trichoderma citrinoviride, Trichoderma aureoviride, Trichoderma longibrachiatum. The appearance frequencies of species Trichoderma harzianum, Trichoderma atrotiviride were very high, 26.0% and 25.2% respectively.